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首页> 外文期刊>Biomaterials Science >Second harmonic generation microscopy of collagen organization in tunable, environmentally responsive alginate hydrogels
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Second harmonic generation microscopy of collagen organization in tunable, environmentally responsive alginate hydrogels

机译：胶原蛋白组织的第二次谐波产生显微镜，环保藻酸盐水凝胶

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We fabricated photocrosslinked, environmentally responsive alginate hydrogels for tissue engineering applications. Methacrylated alginate (ALGMA) hydrogels were prepared across a variety and combination of ionic and covalent (chain growth, step growth, and mixed mode) crosslinking strategies to obtain a range of compressive moduli from 9.3 +/- 0.2 kPa for the softest ionically crosslinked hydrogels to 22.6 +/- 0.3 kPa for the dually crosslinked ionic mixed mode gels. The swelling behavior of the alginate hydrogels was significantly higher under basic pH conditions. Stiffer gels consistently swelled to a lesser degree compared to softer gels for all conditions. These hydrogels were stable - retaining >80% of their original mass for three weeks - when incubated in a basic solution of diluted sodium hydroxide, which mimicked accelerated degradation conditions. Encapsulated NIH/3T3 fibroblasts remained viable and proliferated significantly more in stiffer hydrogel substrates compared to softer gels. Additionally, the collagen secreted by encapsulated fibroblasts was quantifiably compared using second harmonic generation (SHG) imaging. Fibroblasts encapsulated in the softer hydrogels secreted significantly less collagen than the stiffer gels. The collagen in these softer gels was also more aligned than the stiffer gels. The ability to tune collagen organization using hydrogels has potential applications ranging from corneal wound healing where organized collagen is desired to epithelial wound scaffolds where a random organization is preferable.
机译：我们制造了用于组织工程应用的光电容，环保藻酸盐水凝胶。制备甲基丙烯酸丙酸盐（藻类）水凝胶在离子和共价（链生长，步骤生长，混合模式）交联策略的各种和组合中，以获得来自9.3 +/- 0.2kPa的一系列压缩模量，用于最柔软的离子交联的水凝胶对于双重交联离子混合模式凝胶至22.6 +/- 0.3kPa。在碱性pH条件下，藻酸盐水凝胶的溶胀行为显着高。与所有条件的较软凝胶相比，静止凝胶始终膨胀至较小程度。这些水凝胶稳定 - 保持其原始质量的80％，持续三周 - 在稀释的氢氧化钠的碱性溶液中孵育，其模仿加速的降解条件。与软凝胶相比，封装的NIH / 3T3成纤维细胞仍然可容易得到纤维凝胶底物的可行性和增殖。另外，使用封装成纤维细胞分泌的胶原蛋白，使用第二谐波产生（SHG）成像进行比较。包封在更柔软的水凝胶中的成纤维细胞分泌显着较低的胶原蛋白比纤糊凝胶显着较低。这些较软凝胶中的胶原蛋白也比纤巧凝胶更准确。使用水凝胶调谐胶原蛋白组织的能力具有来自角膜伤口愈合的潜在应用，其中所需的组织胶原蛋白是优选随机组织的上皮伤口支架。

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