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Comparative analysis of the cellular entry of polystyrene and gold nanoparticles using the freeze concentration method

机译：聚苯乙烯和金纳米颗粒使用冷冻浓度法的比较分析

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摘要

Despite advances in nanoparticle delivery, established physical approaches, such as electroporation and sonication, result in cell damage, limiting their practical applications. In this study, we proposed a unique freeze concentration-based technique and evaluated the efficacy of the method using two types of nanoparticles: citrate-capped gold nanoparticles and carboxylated polystyrene nanoparticles. We further compared the internalisation behaviour of particles of various sizes with and without freezing. Confocal microscopic images showed that the uptake efficacy of 50 nm nanomaterials was greater than that of 100 nm particles. Polystyrene nanoparticles of 50 nm size had more favourable adsorption and internalisation behaviours compared to those of gold nanoparticles after freeze concentration. We also examined the possible endocytic pathways involved in the uptake of gold and polystyrene nanoparticles, and found that the route differed between non-frozen and frozen conditions. Overall, we determined the influence of the freeze concentration strategy on both nanomaterial internalisation and the endocytic uptake pathway. Our findings provide a mechanistic understanding of the internalisation of nanoparticles using a freezing approach and thereby contribute to further developments in nanotherapeutic applications.
机译：尽管纳米粒子递送进展，但建立了物理方法，如电穿孔和超声，导致细胞损坏，限制了它们的实际应用。在这项研究中，我们提出了一种独特的冻结浓度的技术，并使用两种类型的纳米颗粒评估方法的功效：柠檬酸盖金纳米颗粒和羧化聚苯乙烯纳米颗粒。我们进一步比较了各种尺寸的颗粒的内化行为，而不会冻结。共聚焦微观图像显示50nm纳米材料的摄取功效大于100nm颗粒的摄取功效。与冷冻浓度后的金纳米粒子相比，50nm尺寸的聚苯乙烯纳米颗粒具有更有利的吸附和内化行为。我们还研究了参与金和聚苯乙烯纳米颗粒的吸收中可能的内吞的途径，发现该路径不同于非冷冻和冷冻条件。总的来说，我们确定了冻浓度策略对纳米材料内化和内吞的摄取途径的影响。我们的研究结果提供了使用冷冻方法的纳米颗粒的内化的机械理论理解，从而有助于纳米治疗应用的进一步发展。

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  • 来源
    《Biomaterials Science》 |2018年第7期|共9页
  • 作者

    Ahmed Sana; Okuma Koyo; Matsumura Kazuaki;

  • 作者单位

    Japan Adv Inst Sci &

    Technol Sch Mat Sci Nomi Ishikawa 9231292 Japan;

    Japan Adv Inst Sci &

    Technol Sch Mat Sci Nomi Ishikawa 9231292 Japan;

    Japan Adv Inst Sci &

    Technol Sch Mat Sci Nomi Ishikawa 9231292 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子生物学;
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