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首页> 外文期刊>Biomaterials Science >Transgenic PDGF-BB/sericin hydrogel supports for cell proliferation and osteogenic differentiation
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Transgenic PDGF-BB/sericin hydrogel supports for cell proliferation and osteogenic differentiation

机译：转基因PDGF-BB / Sericin Hydrogel支持细胞增殖和骨质发生分化

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摘要

Sericin has been exploited as a biomaterial due to its biocompatibility, biodegradability, and low-immunogenicity as an isolated polymer and support for cell adhesion. In the present study, human platelet-derived growth factor (PDGF-BB)-functionalized sericin hydrogels were generated using transgenic silkworms, where the as-spun silk incorporated engineered PDGF-BB (termed PDGFM) in the sericin layers of the cocoons. Sericin and PDGFM were simultaneously extracted from the silk fibroin cocoon fibers, and the soluble extract was then formed into a hydrogel via thermal exposure. The PDGFM sericin hydrogels exhibited increased beta-sheet content and a compressive modulus of 74.91 +/- 2.9 kPa comparable to chemically crosslinked sericin hydrogels (1.68-55.53 kPa) and a porous microstructure, which contributed to cell adhesion and growth. A 13.1% of total extracted PDGFM from the initial silk fibers was incorporated and immobilized in the sericin hydrogels during material processing, and 1.33% of PDGFM was released over 30 days from the hydrogels in vitro. The remaining PDGFM achieved long-term storage/stability in the sericin hydrogels for more than 42 days at 37 degrees C. In addition, the PDGFM sericin hydrogels were not immunogenic, were biocompatible and bioactive in promoting the support of cell proliferation. When combined with BMP-9, the PDGFM sericin hydrogels provided synergy to support the osteoblastic differentiation of mesenchymal stem cells (hMSCs) in vitro and in vivo. This study demonstrates that genetically functionalized PDGFM sericin hydrogels can provide useful biomaterials to support cell and tissue outcomes, here with a focus on osteogenesis.
机译：由于其生物相容性，生物相容性和低免疫原性作为分离的聚合物和对细胞粘附的载体而被利用为生物材料。在本研究中，使用转基因蚕产生人的血小板衍生的生长因子（PDGF-BB） - 官能化胺蛋白水凝胶，其中在茧的硅蛋白层中的AS-Spun丝掺入工程化PDGF-BB（称为PDGFM）。从丝素蛋白茧纤维同时提取丝裂蛋白和PDGFM，然后通过热暴露形成可溶性提取物。 PDGFM Sericin水凝胶具有增加的β-片状含量和74.91 +/- 2.9kPa的压缩模量与化学交联的胺水凝胶（1.68-55.53kPa）和多孔微观结构有助于细胞粘附和生长。从初始丝纤维中提取的13.1％的总提取的PDGFM在材料加工过程中掺入并固定在紫苏水凝胶中，并且1.33％的PDGFM在体外从水凝胶30天内释放。其余的PDGFM在37摄氏度下达到霉菌水凝胶中的长期储存/稳定性。此外，PDGFM Sericin水凝胶不是免疫原性，在促进细胞增殖的支持下，生物相容性和生物活性。当与BMP-9结合时，PDGFM Sericin水凝胶提供了协同作用，以支持体外和体内间充质干细胞（HMSCs）的骨细胞分化。该研究表明，遗传官能化的PDGFM Sericin水凝胶可以提供有用的生物材料，以支持细胞和组织结果，这里具有焦点对骨质发生。

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  • 来源
    《Biomaterials Science》 |2020年第2期|共16页
  • 作者

    Wang Feng; Hou Kai; Chen Wenjing; Wang Yuancheng; Wang Riyuan; Tian Chi; Xu Sheng; Ji Yanting; Yang Qianqian; Zhao Ping; Yu Ling; Lu Zhisong; Zhang Huijie; Li Fushu; Wang Han; He Baicheng; Kaplan David L.; Xia Qingyou;

  • 作者单位

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

    Southwest Univ Fac Mat &

    Energy Inst Clean Energy &

    Adv Mat Chongqing 400715 Peoples R China;

    Southwest Univ Fac Mat &

    Energy Inst Clean Energy &

    Adv Mat Chongqing 400715 Peoples R China;

    Chongqing Med Univ Key Lab Biochem &

    Mol Pharmacol Chongqing Chongqing 400016 Peoples R China;

    Chongqing Med Univ Sch Pharm Dept Pharmacol Chongqing 400016 Peoples R China;

    Chongqing Med Univ Sch Pharm Dept Pharmacol Chongqing 400016 Peoples R China;

    Chongqing Med Univ Sch Pharm Dept Pharmacol Chongqing 400016 Peoples R China;

    Tufts Univ Dept Biomed Engn Medford MA 02155 USA;

    Southwest Univ Chongqing Engn &

    Technol Res Ctr Novel Silk Mat Chongqing Key Lab Sericultural Sci Biol Sci Res Ctr Chongqing 400715 Peoples R China;

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  • 中图分类 分子生物学;
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