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首页> 外文期刊>Biomaterials Science >Haemoglobin-loaded metal organic framework-based nanoparticles camouflaged with a red blood cell membrane as potential oxygen delivery systems
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Haemoglobin-loaded metal organic framework-based nanoparticles camouflaged with a red blood cell membrane as potential oxygen delivery systems

机译：加载血红蛋白的金属有机框架的纳米粒子伪装红细胞膜作为潜在的氧气输送系统

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摘要

Transfusion of donor red blood cells (RBCs) is a crucial methodology required for the treatment of acute trauma and anaemia or for surgical procedures. Due to the many limitations of donor blood, numerous strategies have been explored to develop haemoglobin (Hb)-based oxygen carriers to be used as oxygen delivery systems. However, since free Hb suffers from a lack of stability and short circulation times in blood, an encapsulation platform is needed. Herein, we entrap Hb within a type of metal organic framework (MOF)-based nanoparticle (MOF-NP). By doing so, Hb is protected from misfolding and denaturation, which is a crucial aspect to preserve its excellent oxygen binding and releasing properties. Furthermore, the porous structure of MOF-NPs allows for the diffusion of small molecules (i.e., oxygen) in and out of the system. Our results show that the Hb-loaded MOF-NPs (MOFHb-NPs) are monodisperse and show a small hydrodynamic diameter of similar to 220 nm. Importantly, the structure and functionality of the encapsulated Hb are well preserved. To achieve long circulation in the bloodstream, we functionalized MOFHb-NPs with naturally derived RBC membranes and compared the stealth properties of the membrane-coated MOFHb-NPs with our previously reported PEGylation strategy. Protein adsorption and cell uptake studies demonstrate that both coatings are able to significantly decrease the adsorption of proteins and also diminish their uptake by macrophages and endothelial cells. Furthermore, both types of coatings endow MOFHb-NPs with good biocompatibility and oxygen binding and releasing properties. Overall, this study presents a novel oxygen carrier system which might find applications as a blood surrogate.
机译：供体红细胞（RBC）的输血是治疗急性创伤和贫血或外科手术所需的关键方法。由于供体血液的许多局限性，已经探索了许多策略，以发展血红蛋白（HB）基于氧载体作为氧输送系统。然而，由于自由Hb患有缺乏稳定性和血液中的短循环时间，因此需要封装平台。在此，我们在一类金属有机骨架（MOF）的纳米颗粒（MOF-NP）中诱捕HB。通过这样做，Hb被保护免于错误折叠和变性，这是保持其优异的含氧结合和释放性质的关键方面。此外，MOF-NP的多孔结构允许在系统中扩散小分子（即氧气）。我们的结果表明，HB加载的MOF-NPS（MOFHB-NPS）是单分散的，并显示出类似于220nm的小流体动力直径。重要的是，封装HB的结构和功能良好被保存。为了在血液中实现长循环，我们用天然衍生的RBC膜官能化MOFHB-NPS，并将膜涂覆的MOFHB-NPS的隐身性能与我们先前报道的PEG化策略进行了比较。蛋白质吸附和细胞吸收研究表明，两种涂层都能够显着降低蛋白质的吸附，并且还通过巨噬细胞和内皮细胞降低它们的吸收。此外，两种类型的涂层赋予MoFHB-NPS，具有良好的生物相容性和氧合含量和释放性能。总体而言，本研究提出了一种新型氧气载体系统，其可能会发现应用作为血液替代品。

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  • 来源
    《Biomaterials Science》 |2020年第21期|共15页
  • 作者

    Liu Xiaoli; Jansman Michelle M. T.; Hosta-Rigau Leticia;

  • 作者单位

    Tech Univ Denmark Ctr Nanomed &

    Theranost DTU Hlth Tech Nils Koppels Alle B423 DK-2800 Lyngby Denmark;

    Tech Univ Denmark Ctr Nanomed &

    Theranost DTU Hlth Tech Nils Koppels Alle B423 DK-2800 Lyngby Denmark;

    Tech Univ Denmark Ctr Nanomed &

    Theranost DTU Hlth Tech Nils Koppels Alle B423 DK-2800 Lyngby Denmark;

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  • 正文语种 eng
  • 中图分类 分子生物学;
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