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首页> 外文期刊>Biomaterials Science >Electrospun poly(N-isopropyl acrylamide)/poly(caprolactone) fibers for the generation of anisotropic cell sheets
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Electrospun poly(N-isopropyl acrylamide)/poly(caprolactone) fibers for the generation of anisotropic cell sheets

机译：用于产生各向异性细胞片的Electrome淘聚（N-异丙基丙烯酰胺）/聚（己内酯）纤维

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摘要

Cell alignment in muscle, nervous tissue, and cartilage is requisite for proper tissue function; however, cell sheeting techniques using the thermosensitive polymer poly(N-isopropyl acrylamide) (PNIPAAm) can only produce anisotropic cell sheets with delicate and resource-intensive modifications. We hypothesized that electrospinning, a relatively simple and inexpensive technique to generate aligned polymer fibers, could be used to fabricate anisotropic PNIPAAm and poly(caprolactone) (PCL) blended surfaces that both support cell viability and permit cell sheet detachment via PNIPAAm dissolution. Aligned electrospun PNIPAAm/PCL fibers (0%, 25%, 50%, 75%, 90%, and 100% PNIPAAm) were electrospun and characterized. Fibers ranged in diameter from 1-3 mu m, and all fibers had an orientation index greater than 0.65. Fourier transform infrared spectroscopy was used to confirm the relative content of PNIPAAm and PCL. For advancing water contact angle and mass loss studies, only high PNIPAAm-content fibers (75% and greater) exhibited, temperature-dependent properties like 100% PNIPAAm fibers, whereas 25% and 50% PNIPAAm fibers behaved similarly to PCL-only fibers. 3T3 fibroblasts seeded on all PNIPAAm/ PCL fibers had high cell viability and spreading except for the 100% PNIPAAm fibers. Cell sheet detachment by incubation with cold medium was successful only for 90% PNIPAAm fibers, which had a sufficient amount of PCL to allow cell attachment and spreading but not enough to prevent detachment upon PNIPAAm dissolution. This study demonstrates the feasibility of using anisotropic electrospun PNIPAAm/ PCL fibers to generate aligned cell sheets that can potentially better recapitulate anisotropic architecture to achieve proper tissue function.
机译：肌肉，神经组织和软骨中的细胞对齐是正确的组织功能的必要条件;然而，使用热敏聚合物聚（N-异丙烯酰胺）（PNIPAAM）的细胞片技术可以仅生产具有细腻和资源密集型修饰的各向异性细胞片。我们假设静电纺丝，产生对齐的聚合物纤维的相对简单且廉价的技术，可用于制造各向异性泊匹帕和聚（己内酯）（PCL）混合表面，其支持细胞活力并允许通过泊匹普米溶解脱离的细胞片脱离。对齐的ElectromeTh泊匹帕米/ PCL纤维（0％，25％，50％，75％，90％和100％泊匹吡普）是Electrom淘汰的，并表征。直径为1-3μm的纤维，所有纤维的取向指数大于0.65。傅里叶变换红外光谱法用于确认泊匹帕姆和PCL的相对含量。为了推进水接触角和质量损失研究，仅表现出高肺痘样纤维（75％和更高），依赖于100％泊匹帕纤维的温度依赖性性质，而25％和50％泊贝纤维的表现与仅PCL的纤维类似。除了100％泊匹普米纤维外，所有泊腓/ PCL纤维上播种的3T3成纤维细胞具有高细胞活力和扩散。通过与冷培养基孵育的细胞片脱离仅为90％的肺脂纤维成功，其具有足够量的PCL以允许细胞附着和扩散，但不足以防止脱脂溶解时脱离。本研究表明使用各向异性Electropum肺腺/ PCL纤维产生对准的细胞片的可行性，所述对准的细胞片可以潜在地能够促进各向异性架构以实现适当的组织功能。

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