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首页> 外文期刊>Biomaterials Science >Chitosan/PEI patch releasing EGF and the EGFR gene for the regeneration of the tympanic membrane after perforation
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Chitosan/PEI patch releasing EGF and the EGFR gene for the regeneration of the tympanic membrane after perforation

机译：壳聚糖/ PEI蛋白释放EGF和EGFR基因穿孔后鼓膜再生

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摘要

Damage to the eardrum causes acute pain and can lead to chronic otitis media if it develops into chronic tympanic membrane (TM) perforations. Chronic TM perforations are usually treated with surgical methods such as tympanoplasty and myringoplasty. However, these surgeries are not only complicated and difficult but also cost a lot of money. Our research team developed chitosan patches (E-CPs) that release epidermal growth factor (EGF) as a patch therapy to replace surgical methods. However, there was a limitation in the healing ratio of the treatment compared to the surgical methods. In this study, we developed EGF and epidermal growth factor receptor (EGFR) gene-releasing polyethyleneimine (PEI)/schitosan patches (EErP-CPs) to increase the regeneration of TM perforations. The addition of PEI increased the adhesion and migration ability of TM cells on the patches. The simultaneous release of the EGF and the EGFR gene further enhanced TM cell proliferation, adhesion and migratory ability. It was confirmed that the EGF protein and EGFR gene were released for 30 days; however, EGF was released and increased TM cell viability almost immediately after treatment and EGFR took a minimum of 3 days before showing its effect on improved cell viability. It was also shown that EErP-CPs are more hydrophilic and have more positive charge than E-CP because of added amine groups from PEI. In conclusion, the developed EErP-CPs resulted in the improved healing of TM perforations and can potentially be applied to the regeneration of both chronic and acute tympanic membrane perforations.
机译：对耳膜的损伤导致急性疼痛，如果它发展成慢性鼓膜膜（TM）穿孔，可以导致慢性中耳炎。慢性TM穿孔通常用手术方法进行治疗，例如鼓膜成形术和灭弧形成形术。然而，这些手术不仅复杂且困难，而且还花了很多钱。我们的研究团队开发了壳聚糖补丁（E-CPS），将表皮生长因子（EGF）释放为贴剂治疗以取代手术方法。然而，与手术方法相比，治疗的愈合比有一个限制。在该研究中，我们开发了EGF和表皮生长因子受体（EGFR）基因释放聚乙烯亚胺（PEI）/ Schitosan贴剂（EERP-CP）以增加TM穿孔的再生。添加PEI增加了TM细胞对贴剂上的粘附性和迁移能力。 EGF的同时释放和EGFR基因进一步增强了TM细胞增殖，粘附性和迁移能力。证实，EGF蛋白和EGFR基因释放30天;然而，在治疗后几乎立即释放EGF并增加TM细胞活力，并且在展示其对改善细胞活力的影响之前，EGFR至少花费3天。还表明，由于来自PEI的添加胺基，Eerp-CPS更亲水，并且具有比E-CP更高的阳性电荷。总之，发育的EERP-CPS导致TM穿孔的愈合改善，并且可能潜在地应用于慢性和急性鼓膜膜穿孔的再生。

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  • 来源
    《Biomaterials Science》 |2018年第2期|共8页
  • 作者

    Lee Myung Chul; Seonwoo Hoon; Garg Pankaj; Jang Kyoung Je; Pandey Shambhavi; Park Sang Bae; Kim Hong Bae; Lim Jaewoon; Choung Yun Hoon; Chung Jong Hoon;

  • 作者单位

    Seoul Natl Univ Dept Biosyst &

    Biomat Sci &

    Engn Seoul 151742 South Korea;

    Sunchon Natl Univ Dept Ind Machinery Engn 315 Maegok Dong Sunchon South Korea;

    Seoul Natl Univ Res Inst Agr &

    Life Sci Seoul 151921 South Korea;

    Seoul Natl Univ Res Inst Agr &

    Life Sci Seoul 151921 South Korea;

    Seoul Natl Univ Dept Biosyst &

    Biomat Sci &

    Engn Seoul 151742 South Korea;

    Seoul Natl Univ Dept Biosyst &

    Biomat Sci &

    Engn Seoul 151742 South Korea;

    Seoul Natl Univ Dept Biosyst &

    Biomat Sci &

    Engn Seoul 151742 South Korea;

    Ajou Univ Sch Med Dept Otalaryngol Suwon 443749 South Korea;

    Seoul Natl Univ Dept Biosyst &

    Biomat Sci &

    Engn Seoul 151742 South Korea;

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  • 中图分类 分子生物学;
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