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首页> 外文期刊>Biomaterials Science >Polydopamine-assisted one-step modification of nanofiber surfaces with adenosine to tune the osteogenic differentiation of mesenchymal stem cells and the maturation of osteoclasts
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Polydopamine-assisted one-step modification of nanofiber surfaces with adenosine to tune the osteogenic differentiation of mesenchymal stem cells and the maturation of osteoclasts

机译：聚德莫胺辅助纳米纤维表面的一步改性腺苷曲调间充质干细胞的成骨分化及骨壳细胞成熟

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Adenosine and its receptors have emerged as alternative targets to control cellular functions for bone healing. However, the soluble delivery of adenosine has not proven effective because of its fast degradation in vivo. We therefore designed a stable coating of adenosine for biomaterial surfaces through polydopamine chemistry to control osteogenesis and osteoclastogenesis via A2bR signaling. First, we prepared electrospun poly (iota-lactic acid) (PLLA) nanofiber sheets, which were modified through a one-step adenosine polydopamine coating process. Scanning electron microscopy (SEM) revealed deposition of particles on the adenosine polydopamine-coated PLLA (AP-PL) sheets compared to the polydopamine-only sheets. Moreover, X-ray photoelectron spectroscopy analysis confirmed an increase in nitrogen signals due to adenosine. Furthermore, adenosine loading efficiency and retention were significantly enhanced in AP-PL sheets compared to polydopamine-only sheets. Human adipose-derived stem cells (hADSCs) cultured on AP-PL expressed A2bR (1.30 +/- 0.19 fold) at significantly higher levels than those cultured on polydopamine-only sheets. This in turn significantly elevated the expression of Runx2 (16.94 +/- 1.68 and 51.69 +/- 0.07 fold), OPN (1.63 +/- 0.16 and 30.56 +/- 0.25 fold), OCN (1.16 +/- 0.13 and 5.23 +/- 0.16 fold), and OSX (10.01 +/- 0.81 and 62.48 +/- 0.25 fold) in cells grown in growth media on days 14 and 21, respectively. Similarly, mineral deposition was enhanced to a greater extent in the AP-PL group than the polydopamine group, while blocking of A2bR significantly downregulated osteogenesis. Finally, osteoclast differentiation of RAW 264.7 cells was significantly inhibited by growth on AP-PL sheets. However, osteoclast differentiation was significantly stimulated after A2bR was blocked. Taken together, we propose that polydopamine-assisted one-step coating of adenosine is a viable method for surface modification of biomaterials to control osteogenic differentiation of stem cells and bone healing.
机译：腺苷及其受体已成为控制骨愈合细胞功能的替代靶标。然而，由于其体内的快速降解，腺苷的可溶性递送尚未证明是有效的。因此，我们设计了通过聚二胺化学来控制生物材料表面的腺苷稳定涂层，以通过A2BR信号传导控制骨质发生和骨质细胞发生。首先，我们制备了ElectromeOM聚（IOTA-乳酸）（PLLA）纳米纤维片，其通过一步腺苷多己胺涂布方法改性。扫描电子显微镜（SEM）揭示了与仅聚二胺的片材相比的腺苷聚德莫胺涂覆的PLLA（AP-PL）片上的颗粒沉积。此外，X射线光电子能谱分析证实了由于腺苷引起的氮信号的增加。此外，与仅多胺的片材相比，AP-PL板中的腺苷负载效率和保留显着增强。在AP-PL上培养的人脂肪衍生的干细胞（HADSCs）在显着高于培养的诸如多莫多氨酸片上培养的含量明显较高的A2BR（1.30 +/- 0.19倍。这反过来又显着提升了Runx2的表达（16.94 +/- 1.68和51.69 +/- 0.07倍），OPN（1.63 +/- 0.16和30.56 +/- 0.25倍），OCN（1.16 +/- 0.13和5.23 + / - 0.16倍）和OSX（10.01 +/- 0.81和62.48 +/- 0.25倍）分别在第14和21天生长培养基中生长的细胞中。类似地，在AP-PL组比聚二胺基组中提高矿物沉积在更大程度上，同时阻断A2BR显着下调的骨质发生。最后，通过AP-PL板的生长显着抑制了原始264.7细胞的骨细胞分化。然而，在封闭A2BR后，显着刺激了骨细胞分化。我们提出了腺苷的聚二胺辅助一步涂层是一种可行的生物材料表面改性以控制干细胞和骨愈合的成骨分化。

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