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首页> 外文期刊>Biochemistry and Cell Biology >Lactoferrin inhibits melanogenesis by down-regulating MITF in melanoma cells and normal melanocytes
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Lactoferrin inhibits melanogenesis by down-regulating MITF in melanoma cells and normal melanocytes

机译：乳铁蛋白通过黑色素瘤细胞和正常的黑素细胞下调MITF来抑制黑糖素

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摘要

The aim of this study was to evaluate the effect of bovine lactoferrin (bLf) on melanin-producing cells and to elucidate its mechanism of action. We tested the anti-melanogenic effect of bLf on a 3-dimensional cultured pigmentation skin model and confirmed a 20% reduction in pigmentation, suggesting that bLf was transdermally absorbed and it suppressed melanin production. Treatment of human melanoma cells with bLf resulted in a significant, dose-dependent suppression of melanin production. Apo-bLf and holo-bLf suppressed melanogenesis to the same degree as bLf. The key feature behind this anti-melanogenic effect of bLf was the down-regulation of the microphthalmia-associated transcription factor (MITF), leading to the suppression of tyrosinase activity. Treatment with bLf resulted in both decreased expression of MITF mRNA and enhanced degradation of MITF protein. However, the primary effector was enhanced phosphorylation of extracellular signal-regulated kinase (ERK), leading to the phosphorylation and degradation of MITF. Our finding that bLf suppresses melanin production in melanocytes indicates that bLf is a possible candidate for application as a skin-whitening agent.
机译：本研究的目的是评估牛乳铁蛋白（BLF）对黑色素产生细胞的影响，并阐明其作用机制。我们测试了BLF对三维培养的色素沉着皮肤模型的抗批发作用，并证实了色素沉着的降低20％，表明BLF透皮吸收，并抑制了黑色素的产生。用BLF处理人黑色素瘤细胞导致黑色素产生的显着，剂量依赖性抑制。 APO-BLF和HOLO-BLF抑制了素质生成与BLF相同程度。 BLF的这种抗丝瘤作用背后的关键特征是微蛋白相关转录因子（MITF）的下调，导致抑制酪氨酸酶活性。用BLF处理导致MITF mRNA的表达减少，并提高了MITF蛋白的降解。然而，初级效应器增强了细胞外信号调节激酶（ERK）的磷酸化，导致MITF的磷酸化和降解。我们发现BLF抑制黑素细胞的黑色素产生表明BLF是应用作为皮肤美白剂的可能候选者。

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