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首页> 外文期刊>Biochemistry and Cell Biology >Promoting effect of lactoferrin on barrier function and epithelial differentiation of human keratinocytes
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Promoting effect of lactoferrin on barrier function and epithelial differentiation of human keratinocytes

机译：乳蛋叶蛋白对人角蛋白酶细胞的屏障功能和上皮分化的促进作用

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The purpose of this study was to elucidate the effects of bovine lactoferrin on keratinocyte differentiation and barrier function. Addition of bovine lactoferrin to differentiating HaCaT human keratinocytes led to increased transepithelial electrical resistance (TER), a marker of epithelial barrier function. This elevation was followed by upregulation of two differentiation markers, involucrin and filaggrin. The expression level of sterol regulatory element-binding protein-1 was also enhanced by bovine lactoferrin. The lactoferrin-induced upregulation of involucrin and filaggrin expression were confirmed in normal human epidermal keratinocytes (NHEK). Treatment with SB203580, a p38 mitogen-activated protein kinase (MAPK) α inhibitor, impaired the upregulation of involucrin and filaggrin expression in response to lactoferrin. The elevation of p38 MAPK phosphorylation was further enhanced by lactoferrin in the initial stage of differentiation of HaCaT keratinocytes. The findings suggest that bovine lactoferrin promotes epithelial differentiation by a p38-MAPK-dependent mechanism.
机译：本研究的目的是阐明牛乳铁蛋白对角质形成细胞分化和阻隔功能的影响。添加牛乳铁蛋白以区分HaCAT人角蛋白细胞导致转化的促进性电阻（TER），上皮阻挡功能的标志物。这种升级随后是两个分化标志物，其中包含紫蛋白的上调。牛乳铁蛋白还增强了甾醇调节元素结合蛋白-1的表达水平。在正常人体表皮角蛋白细胞（NHEK）中证实了乳蛋白蛋白诱导的有关霉素和叶虫蛋白表达的上调。用SB203580治疗P38丝裂原激活的蛋白激酶（MAPK）α抑制剂，响应于Lactoferrin，损害了蛋白质和叶片表达的上调性和叶片表达。通过乳铁蛋白在HaCAT角蛋白细胞分化的初始阶段进一步增强了P38 MAPK磷酸化的升高。结果表明，牛乳蛋白通过P38-MAPK依赖性机制促进上皮分化。

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