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首页> 外文期刊>Biochemistry and Cell Biology >Mechanistic effect of human umbilical cord blood derived mesenchymal stem cells on the submandibular salivary gland in ovariectomized rats
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Mechanistic effect of human umbilical cord blood derived mesenchymal stem cells on the submandibular salivary gland in ovariectomized rats

机译：人脐血血液衍生间充质干细胞在卵巢切除大鼠颌下唾液腺上的机械效应

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We performed this study to understand the effect of human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) on the submandibular gland after bilateral ovariectomy. For this, 21 adult female rats were distributed equally among 3 groups: the sham-operated group (SHAM); the ovariectomized group (OVX); and the OVX group that received repeated intravenous injections of the hUCB-MSCs (OVX + hUCB-MSCs). We used reverse transcription – PCR to analyze for the gene expression of AQPs 3 , 4 , 5 , and BMP-6 . The cellular localization and expression of human CD105, human CD34, proliferating nuclear antigen (PCNA), single-stranded DNA (ss-DNA), caspase 3, AQP1, and α smooth muscle actin (α-SMA) were determined immunohistochemically. In the OVX group, a significant decrease in the gene expression of AQP3 , AQP4 , and BMP6 , as well as the acinar area % was detected, while area % of granular convoluted tubules (GCTs) showed a significant increase. A significant decrease in area % staining positively for AQP1 and α-SMA was noted. An obvious improvement in the structure of the submandibular gland was demonstrated in the group injected with hUCB-MSCs, as well as a significant increase in the gene expression of AQP3 , AQP4 , and BMP6 . The acinar and GCT area %, as well as the different measured markers, were relatively normal. This demonstrates that E2-deficiency induces structural changes to the submandibular gland. Moreover, a definite amelioration of the structure and function of the submandibular gland was detected after the administration of hUCB-MSCs.
机译：我们进行了这项研究以了解双侧卵巢切除术后患有人脐血血液衍生间充质干细胞（HUCB-MSCs）对颌下腺的影响。为此，21只成年女性大鼠在3组中平均分布：假手术组（假）;卵巢切除术组（OVX）;和接受HUCB-MSCs（OVX + HUCB-MSCs）重复静脉内注射的OVX组。我们使用逆转录 - PCR分析AQPS 3,4,5和BMP-6的基因表达。免疫组化测定了人类CD105，人CD34，增殖核抗原（PCNA），单链DNA（SS-DNA），胱天蛋白3，AQP1和α平滑肌肌动蛋白（α-SMA）的细胞定位和表达。在OVX组中，检测AQP3，AQP4和BMP6的基因表达的显着降低，以及缩醛面积％，而粒状卷曲小管（GCTS）的面积％显示出显着增加。注意到AQP1和α-SMA对阳性染色的面积％显着降低。在注射HUCB-MSC的基团中证明了颌下腺结构的明显改善，以及AQP3，AQP4和BMP6的基因表达的显着增加。缩醛和GCT面积％，以及不同的测量标记相对正常。这表明E2缺乏症诱导颌下腺的结构变化。此外，在施用HUCB-MSCs后检测到潜水腺的结构和功能的明确改善。

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