• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry and Cell Biology >MicroRNA-1-3p inhibits the proliferation and migration of oral squamous cell carcinoma cells by targeting DKK1
【24h】

MicroRNA-1-3p inhibits the proliferation and migration of oral squamous cell carcinoma cells by targeting DKK1

机译：microRNA-1-3P通过靶向DKK1来抑制口腔鳞状细胞癌细胞的增殖和迁移

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We investigated the functional role and mechanism of miR-1-3p and DKK1 in oral squamous cell carcinoma (OSCC) cells. The level of miR-1-3p and DKK1 expression were detected in OSCC tissues and cells using reverse-transcription -quantitative PCR and Western blot. A dual luciferase reporter gene assay was applied to confirm the targeting relationship between miR-1-3p and DKK1. Functional assays, including MTT, Transwell, colony formation, and flow cytometry analysis were conducted to verify their effect on cell progressions. MTT, colony formation, and Transwell assays indicated that the proliferation, migration, and invasion of SCC-4 cells was impaired with high miR-1-3p expression but promoted with high DKK1 expression. The results from cell cycle analysis and annexin-V-PI assays for apoptosis suggested that miR-1-3p suppressed the transit of SCC-4 cells from G0/G1 to S and induced apoptosis. In summary, miR-1-3p suppressed the progression of OSCC by inhibiting DKK1 expression.
机译：我们研究了口腔鳞状细胞癌（OSCC）细胞中miR-1-3p和dkk1的功能作用和机制。 使用反转转录-MCR和Western印迹在OSCC组织和细胞中检测miR-1-3p和DKK1表达水平。 应用双荧光素酶报告基因测定以确认miR-1-3p和DKK1之间的靶向关系。 进行功能测定，包括MTT，Transwell，菌落形成和流式细胞术分析以验证它们对细胞进展的影响。 MTT，菌落形成和Transwell测定表明，SCC-4细胞的增殖，迁移和侵袭患有高miR-1-3P表达损害，但用高DKK1表达促进。 细胞周期分析和吞噬蛋白-V-PI测定的结果表明，miR-1-3p抑制了来自g0 / g1至s的SCC-4细胞的过境并诱导细胞凋亡。 总之，MIR-1-3P通过抑制DKK1表达来抑制OSCC的进展。

著录项

  • 来源
    《Biochemistry and Cell Biology》 |2018年第3期|共10页
  • 作者

    Wang Zhenshi; Wang Jiaolong; Chen Zhihua; Wang Kun; Shi Lianshui;

  • 作者单位

    Nanchang Univ Affiliated Stomatol Hosp Dept Prosthodont Nanchang 330006 Jiangxi Peoples R China;

    Nanchang Univ Affiliated Stomatol Hosp Dept Prosthodont Nanchang 330006 Jiangxi Peoples R China;

    Nanchang Univ Affiliated Hosp 2 Dept Neurosurg Nanchang 330006 Jiangxi Peoples R China;

    Nanchang Univ Affiliated Stomatol Hosp Dept Prosthodont Nanchang 330006 Jiangxi Peoples R China;

    Nanchang Univ Affiliated Stomatol Hosp Dept Prosthodont Nanchang 330006 Jiangxi Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

    OSCC; miR-1-3p; DKK1; SCC-4 cells;

    机译：OSCC;miR-1-3p;dkk1;scc-4细胞;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号