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miR-96 targets SOX6 and promotes proliferation, migration, and invasion of hepatocellular carcinoma

机译：miR-96靶向SOX6并促进肝细胞癌的增殖，迁移和侵袭

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摘要

Recent research suggested that microRNA 96 (miR-96) might function as an oncogene in several types of cancers. Therefore, the purpose of this study was to probe into the mechanism of miR-96 in hepatocellular carcinoma (HCC) cells. HCC tissues and non-tumorous tissues, HCC cell lines, and healthy cell lines were all involved in this study. Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect miR-96 and SOX6 mRNA and protein expressions. The direct regulation of miR96 on SOX6 was confirmed by luciferase reporter assays. Cell proliferation and growth were determined by MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay and colony formation assay. Wound healing and transwell assay were employed for migration and invasion analyses. Finally, SPSS 21.0 and GraphPad 7.0 were applied for statistical analyses. In HCC tissues, miR-96 was highly expressed while SOX6 was lowly expressed. The overexpression of miR-96 reversely inhibited the expression of SOX6, contributing to the promotion of the biological functions of HCC cells. miR-96 could promote cell proliferation, migration, and invasion in HCC by targeting SOX6.
机译：最近的研究表明，MicroRNA 96（miR-96）可能用作几种类型的癌症中的癌基因。因此，本研究的目的是探测肝细胞癌（HCC）细胞中miR-96的机制。 HCC组织和非肿瘤组织，HCC细胞系和健康细胞系均涉及该研究。定量实时PCR（QRT-PCR）和蛋白质印迹用于检测miR-96和Sox6 mRNA和蛋白质表达。通过荧光素酶报告管理分析证实了MIR96对SOX6的直接调节。细胞增殖和生长由MTT（3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2- H-四唑溴铵）测定和菌落形成测定法测定。伤口愈合和Transwell测定用于迁移和入侵分析。最后，申请了SPSS 21.0和GraphPad 7.0进行统计分析。在HCC组织中，MIR-96高表达，而SOX6差别表达。 miR-96的过表达逆转抑制SOX6的表达，有助于促进HCC细胞的生物学功能。 miR-96通过靶向SOX6可以促进HCC中的细胞增殖，迁移和入侵。

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