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首页> 外文期刊>Biochemistry and Cell Biology >AIRE1A might be involved in cyclin B2 degradation in testicular lysates.
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AIRE1A might be involved in cyclin B2 degradation in testicular lysates.

机译：Aire1a可能参与睾丸裂解物的细胞周期蛋白B2降解。

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摘要

The autoimmune regulator gene Aire shows predominant expression in thymus and other immunologically relevant tissues, and is assigned the major function of programming autoreactive T-cell deletion. However, the expression of this gene in tissues outside the immune system raises a question about its possible function beyond the T-cell deletion dogma. We detected Aire in mouse testis, and the expression of AIRE protein was remarkably high in postmeiotic germ cells. Sequencing results indicate that testis expressed Aire variant 1a. AIRE could be detected in spermatozoa, with heavy localization on the principal acrosomal domains. Mouse oocytes stained negatively for AIRE before fertilization, but stained positively for AIRE 30 min after fertilization. In the zygote, the levels of AIRE correlated negatively with cyclin B2 levels. Goat testicular lysates spiked with recombinant human AIRE exhibited augmented cyclin B2 degradation in the presence of protease inhibitors, which was inhibited by MG-132, indicating the operation of proteasomal pathways. Thus, this study identifies a correlation between the presence of AIRE and proteasomal breakdown of cyclin B2, which leads us to speculate that cyclin B2 could be a target of AIRE's E3-ubiquitin ligase activity.
机译：自身免疫调节剂基因AIVE在胸腺和其他免疫相关组织中显示出主要的表达，并分配了编程自身反应性T细胞缺失的主要功能。然而，这种基因在免疫系统之外的组织中的表达提出了关于其可能超出T细胞缺失教条的问题的问题。我们在小鼠睾丸中检测到AIVE，后期胚芽细胞的AIVE蛋白的表达显着高。测序结果表明睾丸表达AIVE变体1a。可以在精子中检测到Aire，在主要的针灸域上具有重定位。在受精之前，小鼠卵母细胞对AIVE染色，但受精后30分钟染色。在Zygote中，AIVE水平与细胞周期蛋白B2水平负相关。山羊睾丸裂解物掺入重组人AiVe的蛋白质B2在蛋白酶抑制剂存在下，其被Mg-132抑制，表明蛋白酶体途径的操作。因此，该研究确定了细胞周期蛋白B2的Aire和蛋白酶体分解之间的相关性，这导致我们推测Cyclin B2可以是Aire的E3-泛素连接酶活性的目标。

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