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首页> 外文期刊>Biochemistry and Cell Biology >Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase
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Capacity of ceruloplasmin to scavenge products of the respiratory burst of neutrophils is not altered by the products of reactions catalyzed by myeloperoxidase

机译：Myeloperoxid酶催化的反应产物没有改变刺激性呼吸爆发的呼吸爆发产物的能力

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摘要

CP is a copper-containing ferroxidase of blood plasma, which acts as an acute phase reactant during inflammation. The effect of oxidative modification of CP induced by oxidants produced by MPO, such as HOCI, HOBr, and HOSCN, on its spectral, enzymatic, and anti-inflammatory properties was studied. We monitored the chemiluminescence of lucigenin and luminol along with fluorescence of hydroethidine and scopoletin to assay the inhibition by CP of the neutrophilic respiratory burst induced by PMA or fMLP. Superoxide dismutase activity of CP and its capacity to reduce the production of oxidants in respiratory burst of neutrophils remained virtually unchanged upon modifications caused by HOCI, HOBr, and HOSCN. Meanwhile, the absorption of type I copper ions at 610 nm became reduced, along with a drop in the ferroxidase and amino oxidase activities of CP. Likewise, its inhibitory effect on the halogenating activity of MPO was diminished. Sera of either healthy donors or patients with Wilson disease were co-incubated with neutrophils from healthy volunteers. In these experiments, we observed an inverse relationship between the content of CP in sera and the rate of H2O2 production by activated neutrophils. In conclusion, CP is likely to play a role of an anti-inflammatory factor tempering the neutrophil respiratory burst in the bloodstream despite the MPO-mediated oxidative modifications.
机译：CP是血浆的含铜铁脂酶，其在炎症期间用作急性相反应物。通过MPO，如Hoci，Hoci，Hoci，HOCR和Hostn产生的氧化剂诱导的CP诱导的CP氧化改性在其光谱，酶和抗炎性质上进行了研究。我们监测含霉素和鲁米诺的化学发光以及氢丙酮和微量蛋白的荧光，以通过PMA或FMLP诱导的嗜中性呼吸脉冲裂的Cp抑制。 CP的超氧化物歧化酶活性及其减少呼吸爆发中呼吸爆发中的氧化剂的产能在由Hoci，Hoci，Hoci，Hoci，Hoci和Hostn引起的修饰后几乎不变。同时，在610nm处的I型铜离子的吸收变为减少，并且CP的铁氧基酶和氨基氧化酶活性的下降。同样，其对MPO的卤化活性的抑制作用减少了。健康供体或威尔逊病患者的血清与来自健康志愿者的中性粒细胞共育。在这些实验中，我们观察到血清中CP含量与活性中性粒细胞的H2O2产生速率之间的反比关系。总之，尽管MPO介导的氧化修饰，CP可能在血液中发挥抗炎因子回火的作用。

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