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首页> 外文期刊>Biological research for nursing >Oxytocin Levels in Community-Collected Saliva Samples Transported by Dry Versus Wet Ice
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Oxytocin Levels in Community-Collected Saliva Samples Transported by Dry Versus Wet Ice

机译：通过干燥与湿冰运输的社区收集的唾液样品中的催产素水平

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Oxytocin (OT), a neuropeptide produced primarily in the hypothalamus, is associated with both critical physiological and psychological processes, particularly stress and feelings of affiliation. Increasingly, researchers are seeking ways to reliably incorporate OT as an outcome biomarker in clinical research. Previously, OT levels were measured in plasma or urine. Recently, researchers have measured this biomarker in saliva, particularly when conducting research in clinical and community settings. In spite of increased interest in the use of salivary OT in clinical research, procedures for handling, transport, and analysis of specimens vary. It is not known if significant OT protein degradation occurs if samples are initially transported on wet ice before being frozen. The aim of this study is to evaluate the effect of transport media (wet vs. dry ice) on OT levels derived from saliva collected from 12 postpartum women residing in the community. Saliva collected from each participant was divided between two microcentrifuge tubes (MIDSCI, Valley Park, MO), one placed on wet ice and one on dry ice for transport from the participant's home to the laboratory freezer. Time from collection to storage freezer was recorded. Laboratory personnel, blinded to method of transport, batch processed the samples. No significant differences in OT levels were found by transport method. Despite large interperson variations in OT levels, there were negligible intraperson variations. Although further research is required to identify factors (including transport time) related to interperson variation, this study supports the use of wet ice as a means of transporting salivary OT specimens in community-based research.
机译：催产素（OT），一种主要在下丘脑中产生的神经肽，与关键的生理和心理过程，特别是压力和隶属关系有关。研究人员越来越多地寻求可靠地将OT作为临床研究中的结果生物标志物。以前，在血浆或尿液中测量OT水平。最近，研究人员在唾液中测量了这种生物标志物，特别是在临床和社区环境中进行研究时。尽管对临床研究中使用唾液OT的兴趣增加，但样品的处理，运输和分析的程序变化。如果在冷冻之前最初在湿冰上在湿冰上运输样品，则不知道如果发生显着的OT蛋白质降解，则不知道。本研究的目的是评估来自驻留在社区的12个产后妇女的唾液中的转运培养基（湿法与干冰）对唾液的影响。从每个参与者收集的唾液分为两个微量离心管（Midscifuge管（Midsci，Valley Park，Mo）之间，一个放在湿冰上，一个在干冰上，用于从参与者的家到实验室冷冻机运输。记录了从收集到存储冰箱的时间。实验室人员，蒙蔽了运输方法，批量加工样品。通过运输方法发现OT水平没有显着差异。尽管OT水平的中间人变化很大，但缺随的内部内部变化可以忽略不计。虽然需要进一步研究识别与人工单变化相关的因素（包括运输时间），但该研究支持使用湿冰作为在基于社区的研究中运输唾液OT标本的方法。

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