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首页> 外文期刊>Cytokine >Pharmacologic doses of interleukin 8 suppositories induce follicular maturation in rabbits.
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Pharmacologic doses of interleukin 8 suppositories induce follicular maturation in rabbits.

机译：白介素8栓剂的药理剂量可诱导兔卵泡成熟。

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摘要

Interleukin 8 (IL-8) is a neutrophil chemoattractant/activating factor that plays a role in the ovarian physiology leads to investigate the effects of IL-8 on follicular maturation. Experiments were conducted using suppositories containing 100 ng, 200 ng, 400 ng IL-8, 500 microl Witepsol-base (control), human menopausal gonadotropin (im) and conjugate of fluorescein isithiocyanate-labelled IL-8. The levels of IL-8 in ovarian fluid were also measured. Histology of ovaries treated with 200 ng IL-8 showed large antral follicles filled with follicular fluid. The theca layer was divided into an interna and an externa with large extracellular spaces. The granulosa cells were loosened and appeared to be detaching from the granulosa layer. Neutrophils were localized predominantly in the theca and medulla (P<0.0001, P<0.004), and relative collagen concentration was significantly decreased in ovaries of 200 ng IL-8 (P<0.0001) compared with controls. The IL-8 was detected in ovarian fluid after 6 h (P<0.0001), 12 h (P<0.001), and 18 h (P<0.01) compared with 0 h. Fluorescein isithiocyanate-labelled IL-8 conjugate was seen in the follicular wall and endometrium. We conclude that pharmacological dosage of exogenous IL-8 exerts an effect on follicular maturation through granulocyte chemotaxis and activation.
机译：白细胞介素8（IL-8）是嗜中性粒细胞趋化因子/激活因子，在卵巢生理中起作用，导致研究IL-8对卵泡成熟的影响。使用包含100 ng，200 ng，400 ng IL-8、500微升Witepsol碱（对照），人更年期促性腺激素（im）和荧光素异硫氰酸酯标记的IL-8缀合物的栓剂进行实验。还测量了卵巢液中的IL-8水平。用200 ng IL-8处理的卵巢的组织学表现为充满卵泡液的大肛门卵泡。卵泡膜层分为内部和外部，细胞外间隙较大。颗粒细胞松弛并且似乎从颗粒层分离。中性粒细胞主要定位在卵泡膜和髓质中（P <0.0001，P <0.004），与对照组相比，在200 ng IL-8的卵巢中相对胶原浓度显着降低（P <0.0001）。与0 h相比，在6 h（P <0.0001），12 h（P <0.001）和18 h（P <0.01）后的卵巢液中检测到IL-8。在卵泡壁和子宫内膜中可见荧光素异硫氰酸酯标记的IL-8偶联物。我们得出结论，外源性IL-8的药理剂量通过粒细胞趋化作用和激活作用对卵泡成熟产生影响。

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