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首页> 外文期刊>Cytokine >Involvement of alpha-2-macroglobulin receptor in clearance of interleukin 8-alpha-2-macroglobulin complexes by human alveolar macrophages.
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Involvement of alpha-2-macroglobulin receptor in clearance of interleukin 8-alpha-2-macroglobulin complexes by human alveolar macrophages.

机译：α-2-巨球蛋白受体参与人肺泡巨噬细胞清除白介素8-α-2-巨球蛋白复合物的过程。

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The purpose of this study was to determine if interleukin 8 (IL-8) in complex with alpha2-macroglobulin (alpha-2-M) can be taken up by human alveolar macrophages. First, we demonstrated that human alveolar macrophages have receptors for alpha-2-M but not IL-8. The binding of(125)I-labeled alpha-2-M to the cells was specific and saturable, whereas(125)I-labeled recombinant human IL-8 (rhIL-8) did not bind to macrophages. However,(125)I-rhIL-8-alpha-2-M complexes bound to macrophages, and unlabeled alpha-2-M competed for the binding. We then cultured the cells in the presence of(125)I-rhIL-8-alpha-2-M complexes,(125)I-rhIL-8 alone or buffer for 24 h. Macrophages were lysed, and the released radioactivity measured. IL-8 concentrations in supernatants and cells were also measured using an IL-8 ELISA. When the macrophages were incubated with(125)I-rhIL-8-alpha-2-M complexes there was a significant amount of IL-8 associated with the cells. However, this was not the case when the cells were incubated with(125)I- rhIL-8 alone suggesting that only these complexes were taken-up by human alveolar macrophages. Furthermore, the clearance of complexes was specifically inhibited by a monoclonal antibody against the 515-kDa subunit of the alpha-2-M receptor (alpha-2-MR) but not by an isotopic mouse IgG1. The study shows an important clearance mechanism for IL-8 in the lung. Copyright 2000 Academic Press.
机译：这项研究的目的是确定白细胞介素8（IL-8）与α2-巨球蛋白（α-2-M）的复合物是否可以被人类肺泡巨噬细胞摄取。首先，我们证明了人类肺泡巨噬细胞具有α-2-M受体，但没有IL-8受体。 （125）I标记的alpha-2-M与细胞的结合是特异性和饱和的，而（125）I标记的重组人IL-8（rhIL-8）不与巨噬细胞结合。然而，（125）I-rhIL-8-alpha-2-M配合物绑定到巨噬细胞，和未标记的alpha-2-M竞争结合。然后，我们在（125）I-rhIL-8-alpha-2-M复合物，单独（125）I-rhIL-8或缓冲液存在下培养细胞24小时。裂解巨噬细胞，并测量释放的放射性。还使用IL-8 ELISA测量上清液和细胞中的IL-8浓度。当巨噬细胞与（125）I-rhIL-8-alpha-2-M复合物孵育时，有大量与细胞相关的IL-8。然而，当细胞仅与（125）I-rhIL-8一起孵育时情况并非如此，这表明人肺泡巨噬细胞仅摄取这些复合物。此外，复合物的清除受到抗α-2-M受体（515-2-MR）515-kDa亚基的单克隆抗体的特异性抑制，但不受同位素小鼠IgG1的抑制。该研究显示了肺中IL-8的重要清除机制。版权所有2000学术出版社。

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