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Meso-Raman approach for rapid yeast cells identification

机译:中间拉曼快速酵母细胞鉴定方法

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摘要

An increasing effort is currently devoted to developing Raman spectroscopy for identification of microorganisms. Micro-Raman setups are typically used for this purpose with the limit that the intra-species and inter-species spectral variability are comparable, thus limiting the identification capability. To overcome this limit a meso-Raman approach is here implemented. Thin films of planktonic cells are analyzed throughout the collection of back-scattered light providing a Raman signal already averaged over tens of cells. The collecting of unpolarized (VU) and depolarized (HV) Raman signals increased the spectral information obtainable from the data, demonstrating the ability of the principal component analysis to differentiate the most common Candida species, namely C. glabrata, C. albicans, C. parapsilosis and C. tropicalis. The proposed method can contribute to bring Raman spectroscopy closer to its potential clinical use for fast identification of yeast cells.
机译:目前致力于开发Raman光谱的越来越努力,以鉴定微生物。 微拉曼设置通常用于此目的,限制物种内和物种间光谱可变性相当,因此限制了识别能力。 为了克服这一限制,这里有一个中间拉曼方法。 在整个背部散射光的集合中分析浮鳞细胞的薄膜,提供已经在数十个细胞上平均的拉曼信号。 非偏振(VU)和去极化(HV)拉曼信号的收集增加了可从数据获得的光谱信息,证明了主要成分分析的能力,以区分最常见的念珠菌物种,即C.Glabrata,C. albicans,C。 甲状腺素和C.Tropicalis。 该方法可以有助于使拉曼光谱更接近其潜在的临床用途,以便快速鉴定酵母细胞。

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