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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Detection of Mg2+-dependent, coaxial stacking rearrangements in a bulged three-way DNA junction by single-molecule FRET
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Detection of Mg2+-dependent, coaxial stacking rearrangements in a bulged three-way DNA junction by single-molecule FRET

机译：通过单分子卷进行凸出的三元DNA结的Mg2 +依赖性，同轴堆叠重排

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摘要

Three-way helical junctions (3WJs) arise in genetic processing, and they have architectural and functional roles in structured nucleic acids. An internal bulge at the junction core allows the helical domains to become oriented into two possible, coaxially stacked conformers. Here, the helical stacking arrangements for a series of bulged, DNA 3WJs were examined using ensemble fluorescence resonance energy transfer (FRET) and single-molecule FRET (smFRET) approaches. The 3WJs varied according to the GC content and sequence of the junction core as well as the pyrimidine content of the internal bulge. Mg2+ titration experiments by ensemble FRET show that both stacking conformations have similar Mg2+ requirements for folding. Strikingly, smFRET experiments reveal that a specific junction sequence can populate both conformers and that this junction undergoes continual interconversion between the two stacked conformers. These findings will support the development of folding principles for the rational design of functional DNA nanostructures.
机译：在遗传加工中出现三通螺旋连接（3WJS），它们具有结构化核酸的建筑和功能作用。结芯处的内部凸起允许螺旋结构域定向成两个可能的同轴堆叠的塑壳。这里，使用集合荧光共振能量转移（FRET）和单分子FRET（SMFRET）方法检查一系列凸出的DNA 3WJS的螺旋堆叠装置。 3WJS根据结核的GC含量和结核的序列以及内部凸起的嘧啶含量而变化。 MG2 +滴定实验通过集合FRET显示堆叠构象的既有折叠相似的MG2 +要求。尖锐的实验表明，特定的结序列可以填充塑造剂，并且该结在两个堆叠的统一剂之间经历连续的相互互连。这些调查结果将支持折叠原则的折叠原则，了解功能性DNA纳米结构的合理设计。

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