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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Differential effect of multiple kinesin motors on run length, force and microtubule binding rate
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Differential effect of multiple kinesin motors on run length, force and microtubule binding rate

机译：多个Kinesin电动机对运行长度，力和微管结合率的差异效果

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The in vitro transport of cargo by motor proteins constitutes a model system to understand mechanisms of vesicle trafficking inside cells. Here we apply the classic bead assay with a short, stiff kinesin protein to test the effect of multiple motors on essential transport parameters: distance, force and microtubule binding rate. Measurements of unloaded run length show that the transition from single- to multiple-motor behavior can be characterized by the appearance of extended runs, in accordance with a recently proposed model that quantifies the probability of multiple-motor engagement. In this transition, application of mechanical load using optical tweezers allows us to register maximum force values above single kinesin levels (8 pN). Yet, averages of run length and maximum force undergo little change as the probability of multiple-motor participation increases. In contrast, the measured rate of bead binding to microtubules scales linearly with the average number of motors per bead. These observations suggest that multiple motors bound randomly to the same cargo mainly increase the probability of attachment of these cargoes to the cytoskeletal filament network.
机译：通过电机蛋白质的货物的体外运输构成了理解细胞内部囊泡机制的模型系统。在这里，我们用短的僵硬的Kinesin蛋白涂抹经典的珠子测定，以测试多个电动机对基本传输参数的影响：距离，力和微管结合率。卸载运行长度的测量表明，根据最近提出的模型，可以通过延长运行的外观来表征从单个到多电机行为的转换，这些模型量化了多电动机接合概率。在这种转变中，使用光学镊子的机械负荷允许我们注册高于单胰岛水平的最大力值（8 pn）。然而，随着多电机参与的概率增加，运行长度和最大力的平均值几乎没有变化。相反，与微管的珠子结合的测量速率与每珠的平均电动机数线性缩放。这些观察结果表明，随机地约为同一货物的多个电动机主要增加这些货物附着到细胞骨骼灯丝网络的可能性。

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