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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Naive balance between structural stability and DNA-binding ability of c-Myb R2R3 under physiological ionic conditions
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Naive balance between structural stability and DNA-binding ability of c-Myb R2R3 under physiological ionic conditions

机译：在生理离子条件下C-MYB R2R3结构稳定性和DNA结合能力之间的天真平衡

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摘要

The minimum functional unit of c-Myb for specific DNA binding, c-Myb R2R3, fluctuates largely in solution which is critical for its DNA-binding function. The thermal stability of R2R3 increases with increasing NaCl concentrations and upon DNA binding as well. The DNA-binding affinity of R2R3 is stringently dependent on NaCl concentration, and decreases both with an increase or decrease in NaCl concentration from the physiological levels. The decreased DNA-binding affinity under low NaCl concentrations is because of the unfavorable entropy change, in contrast to the thermodynamic contribution under high NaCl concentrations. Together with the findings from thermal stability and structure analyses, the results indicate that R2R3 fluctuates more largely under low ionic strength than high ionic strength, resulting in its decreased stability and DNA-binding affinity. Under physiological conditions, R2R3 fluctuates properly to express its DNA-binding ability. The present results clearly show the naive balance between structural stability and DNA binding.
机译：对于特异性DNA结合，C-MYB R2R3的C-MYB的最小功能单元主要在溶液中波动，这对于其DNA结合功能至关重要。 R2R3的热稳定性随着NaCl浓度的增加和DNA结合而增加。 R2R3的DNA结合亲和力严格依赖于NaCl浓度，并且随着生理水平的增加或降低NaCl浓度而降低。低NaCl浓度下的DNA结合亲和力降低是由于不利的熵变化，与高NaCl浓度下的热力学贡献相反。与热稳定性和结构分析的结果一起，结果表明，R2R3比高离子强度更大的低离子强度波动，导致其降低的稳定性和DNA结合亲和力。在生理条件下，R2R3适当地波动以表达其DNA结合能力。本结果清楚地显示了结构稳定性和DNA结合之间的天真平衡。

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