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Biobanking of Fresh-Frozen Cancer Tissue: RNA Is Stable Independent of Tissue Type with Less Than 1 Hour of Cold Ischemia

机译：新鲜冷冻癌组织的生物库：RNA与组织型稳定，无小于1小时的冷缺血

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Background: The effects of preanalytical variables in tissue processing and storage periods on RNA quality of tissues have been well documented in each type of cancer. However, few studies have been performed on a comparative assessment of the impacts across different cancer tissues, even though it is well known that RNase activity is highly variable in various tissue types and RNase-rich tissues have been found to yield low-quality RNA. Methods: We investigated the impacts of cold ischemia times and long-term storage on RNA integrity in various types of cancer tissue, which had been fresh-frozen and collected at the Samsung Medical Center Biobank. RNA quality was also evaluated with regard to histopathological variables. We analyzed RNA integrity number (RIN) data, which had been obtained from our quality control (QC) processes over the last 7 years. Approximately 2% of samples were randomly selected and processed to measure RIN quarterly and after 6 years of storage for QC purposes. Results: Fresh-frozen tumor tissues yielded high-quality RNA regardless of tumor type and histopathological features. Up to 1-hour cold ischemia times and up to 6-year storage times did not adversely influence RNA integrity. Only 3 samples showed RIN of Conclusions: Tissue quality was not adversely affected by long-term storage or limited variations of cold ischemia times. The low-quality samples could be correlated with the structural composition or intratumoral heterogeneity of tissues. The strict application of standardized protocols for tissue collection is the key for high-quality biobanking.
机译：背景：每种类型的癌症都记录了组织处理和组织处理和储存周期的效果。然而，即使众所周知，已经对不同癌组织跨越不同癌组织的影响的比较评估已经进行了很少的研究，即使众所周知，RNase活性在各种组织类型中是高度可变的，并且已经发现RNase的组织产生低质量的RNA。方法：我们研究了冷缺血时间和长期储存对各种类型的癌症组织的RNA完整性的影响，该组织在三星医学中心Biobank中被新鲜冷冻并收集。还考虑了组织病理学变量评估了RNA质量。我们分析了过去7年来从我们的质量控制（QC）流程中获得的RNA完整性数（RIN）数据。大约2％的样品被随机选择并加工以测量QC季度和6年后的QC目的。结果：无论肿瘤类型和组织病理学特征，新鲜冷冻肿瘤组织产生高质量的RNA。高达1小时的冷缺血时间，最多6年的储存时间没有对RNA完整性产生不利影响。只有3个样品显示出结论：组织质量不会受到长期储存或冷缺血时间有限变化的不利影响。低质量样品可以与组织的结构组成或妥善异质性相关。严格适用于组织收集的标准化协议是高质量生物库的关键。

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