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首页> 外文期刊>Biopreservation and biobanking >New Approach to Cryopreservation of Primary Noncultivated Human Umbilical Vein Endothelium in Biobanking
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New Approach to Cryopreservation of Primary Noncultivated Human Umbilical Vein Endothelium in Biobanking

机译：生物库中原发性非经济人脐静脉内皮的新方法

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It is widely accepted that endothelial dysfunction (ED) is a common feature and a risk factor for cardiovascular diseases and metabolic disorders. Cultures of human umbilical vein endothelial cells (HUVECs) are routinely used in cell-based models to study in vitro molecular and cellular mechanisms of development of different aspects of ED. The methods of the HUVEC extraction and expansion are well developed and standardized. However, when large collections of samples are needed for certain projects, or when samples from a rare population of patients should be collected for future experimental use, HUVEC samples should be transferred to a biobank to be saved in liquid nitrogen for a long period of time until the required collection is completed. This scenario is not always convenient since it requires a lot of effort, a large quantity of expensive culture reagents with limited expiration periods, and sometimes special facilities and well-trained cell biologists among the biobank staff. In this project, we evaluated a method of HUVEC cryopreservation, where the stage of cell culturing and expansion before the transfer of samples to the biobank is eliminated. A total of 55 samples of umbilical cord (UC) were obtained from women immediately after delivery. A primary endothelium pellet derived from 17 UC samples was isolated, frozen, and placed in long-term storage in a liquid nitrogen freezer. Other samples were used to obtain HUVEC cultures. We have demonstrated that cryopreservation of primary endothelium pellets from UC veins without culturing and expansion steps does not affect the physiological features of HUVECs. This new approach would improve the efficiency of biobanking logistics, especially in the case of banking of large collections of endothelial samples.
机译：众所周知，内皮功能障碍（ED）是心血管疾病和代谢障碍的常见特征和危险因素。人脐静脉内皮细胞（HUVECS）的培养物常规用于基于细胞的模型，以研究ED的不同方面的体外分子和细胞机制。 HUVEC提取和扩张的方法均发育良好和标准化。但是，当某些项目需要大量样品时，或者应该收集来自罕见患者群体的样品，以便将来的实验使用，Huvec样品应转移到生物内部省份在液氮中长时间保存在液氮中直到完成所需的集合。这种情况并不总是很方便，因为它需要很多努力，大量昂贵的培养试剂，到期时期有限，有时是Biobank工作人员之间的特殊设施和训练有素的细胞生物学家。在该项目中，我们评估了一种Huvec冷冻保存的方法，其中消除了在将样品转移到BioBank之前的细胞培养和膨胀的阶段。在递送后立即从女性中获得总共55个脐带（UC）样本。将来自17个UC样品的主要内皮颗粒分离，冷冻，并置于液氮冰柜中的长期储存中。其他样品用于获得HUVEC培养物。我们已经证明，来自UC静脉的原发性内皮颗粒的冷冻保存不培养和膨胀步骤不会影响HUVECS的生理特征。这种新方法将提高生物管理物流的效率，特别是在银行的内皮样本的银行业的情况下。

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