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首页> 外文期刊>Biopreservation and biobanking >The Effect of Liposome Treatment on Hemorheology and Metabolic Profile of Human Red Blood Cells During Hypothermic Storage
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The Effect of Liposome Treatment on Hemorheology and Metabolic Profile of Human Red Blood Cells During Hypothermic Storage

机译：脂质体处理对低温储存期间人红细胞血液流变学和代谢谱的影响

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Background:Ex vivo cold storage of red blood cells (RBCs) for transfusion has long been associated with hypothermic storage lesions. It has been proposed that liposomes can be used to mitigate hemorheological elements of RBC membrane storage lesions. This study aimed to determine the appropriate liposome treatment time and assess the effects of liposome treatment on RBC's hemorheological and metabolic profiles. Materials and Methods: Unilamellar liposomes were synthesized to contain a bilayer of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC):cholesterol (7:3mol%). Packed human RBCs (n=4) were divided into untreated control (HEPES-NaCl solution) and liposome-treated samples (2mM DOPC liposomes) and treated at days 2, 21, and 42 of hypothermic storage. RBC quality assessment included percent hemolysis, deformability, aggregation, hematological indices, microvesiculation, supernatant potassium, adenosine triphosphate (ATP), and 2,3-diphosphoglycerate (2,3-DPG). Results: Among the parameters affected by liposome treatment time were deformability, aggregation amplitude (Amp), mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and microparticle mean fluorescence intensity. After 6 weeks of storage, aggregation index (AI) and Amp were significantly increased in liposome-treated RBCs (AI: 45.381.92% vs. 41.54 +/- 4.10%, p=0.020; Amp: 16.38 +/- 2.17 arbitrary units [au] vs. 12.22 +/- 3.29au, p=0.019). Despite comparable hemolysis levels at 3 and 6 weeks, DOPC-treated RBCs showed significantly increased potassium levels for the same time points (3 weeks: 31.2 +/- 2.7mmol/L vs. 30.8 +/- 2.7mmol/L, p=0.007; 6 weeks: 45.0 +/- 3.0mmol/L vs. 43.8 +/- 3.4mmol/L, p=0.013). ATP and 2,3-DPG levels were comparable throughout storage. Conclusions: Liposome treatment seemed to be more beneficial when performed at the beginning of storage up to day 21. DOPC liposome treatment resulted in an improvement in human RBC hemorheology upon storage, with no significant impac
机译：背景：用于输血的红细胞（RBC）的exVi​​vo冷储存长期与低温储存病变有关。已经提出，脂质体可用于减轻RBC膜储存病变的血液流变能量。本研究旨在确定适当的脂质体治疗时间，并评估脂质体处理对RBC血液流变学和代谢谱的影响。合成材料和方法：合成单层脂质体以含有1,2-Dioleya-sn-甘油-3-普啉啉（DOPC）的双层（DOPC）：胆固醇（7：3mol％）。将包装的人RBC（n = 4）分为未处理的对照（Hepes-NaCl溶液）和脂质体处理的样品（2mM DOPC脂质体），并在2,21,21和42天进行低温储存处理。 RBC质量评估包括溶血，可变形性，聚集，血液学指数，微升压，上清液，腺苷三磷酸（ATP）和2,3-二磷酰基（2,3-DPG）。结果：受脂质体处理时间影响的参数是可变形性，聚集幅度（AMP），平均血红蛋白，平均碎石血红蛋白浓度和微粒平均荧光强度。在储存6周后，脂质体处理的RBCs（AI：45.381.92％vs.41.54 +/- 4.10％，P = 0.020; AMP：16.38 +/- 2.17任意单位[Au]与12.22 +/- 3.29au，p = 0.019）。尽管3至6周的溶血水平相当，但DOPC处理的RBCs在相同的时间点显示出显着增加的钾水平（3周：31.2 +/- 2.7mmol / L vs.30.8 +/- 2.7mmol / L，P = 0.007 ; 6周：45.0 +/- 3.0mmol / L与43.8 +/- 3.4mmol / L，P = 0.013）。 ATP和2,3-DPG水平在整个储存过程中都是相当的。结论：脂质体治疗似乎在储存开始到第21天开始时更有益。DOPC脂质体处理导致储存后的人RBC血液流变学改善，没有显着的IMPAC

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