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首页> 外文期刊>Biopreservation and biobanking >Measurement of Specific Heat and Crystallization in VS55, DP6, and M22 Cryoprotectant Systems With and Without Sucrose
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Measurement of Specific Heat and Crystallization in VS55, DP6, and M22 Cryoprotectant Systems With and Without Sucrose

机译：在VS55，DP6和M22冷冻保护系统中测量具有和不含蔗糖的特定热和结晶

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摘要

Cryopreservation represents one if not the only long-term option for tissue and perhaps future organ banking. In one particular approach, cryopreservation is achieved by completely avoiding ice formation (or crystallization) through a process called vitrification. This ice-free approach to tissue banking requires a combination of high-concentration cryoprotective additives such as M22 (9.4M), VS55 (8.4M), or DP6 (6M) and sufficiently fast rates of cooling and warming to avoid crystallization. In this article, we report the temperature-dependent specific heat capacity of the above-mentioned cryoprotective additives in small volumes (10mg sample pans) at rates of 5 degrees C/min and 10 degrees C/min using a commercially available differential scanning calorimetry (TA Instruments Q1000), in the temperature range of -150 degrees C to 30 degrees C. This data can be utilized in heat-transfer models to predict thermal histories in a cryopreservation protocol. More specifically, the effects of temperature dependence of specific heat due to the presence of three different phases (liquid, ice, and vitreous phase) can dramatically impact the thermal history and therefore the outcome of the cryopreservation procedure. The crystallization potential of these cryoprotectants was also investigated by studying cases of maximal and minimal crystallization in VS55 and DP6, where M22 did not crystallize under any rates tested. To further reduce crystallization in VS55 and DP6, a stabilizing sugar (sucrose) was added in varying concentrations (0.15M and 0.6M) and was shown to further reduce crystallization, particularly in VS55, at modest rates of cooling (1 degrees C/min, 5 degrees C/min, and 10 degrees C/min).
机译：冷冻保存代表一个，如果不是组织的唯一长期选择，也许是未来的器官银行业务。在一种特定的方法中，通过完全避免通过称为玻璃化的方法避免冰形成（或结晶）来实现冷冻保存。这种无冰的组织银行方法需要一种高浓缩冷冻保护添加剂，例如M22（9.4M），VS55（8.4M）或DP6（6M），以及充分快速的冷却速度，以避免结晶。在本文中，我们使用市售的差分扫描量热法（ TA仪器Q1000），在-150摄氏度至30℃的温度范围内。该数据可用于传热模型中以预测冷冻保存协议中的热历史。更具体地，特定热量的温度依赖性的影响由于三种不同阶段（液体，冰和玻璃阶段）的存在，可以显着影响热历史，从而影响冷冻保存程序的结果。还通过研究VS55和DP6中的最大和最小结晶的病例来研究这些冷冻保护剂的结晶电位，其中M22在测试的任何速率下没有结晶。为了进一步减少Vs55和DP6中的结晶，以不同浓度（0.15m和0.6m）加入稳定糖（蔗糖），并显示进一步减少结晶，特别是在Vs55中，以适度的冷却（1℃/ min） ，5℃/ min和10℃/ min）。

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