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首页> 外文期刊>Biopreservation and biobanking >Effect of Cryopreservation on Lipid Peroxidation, Antioxidant Potential, Chromatin Integrity, and Mitochondrial Activity of Indian Red Jungle Fowl (Gallus gallus murghi) Semen
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Effect of Cryopreservation on Lipid Peroxidation, Antioxidant Potential, Chromatin Integrity, and Mitochondrial Activity of Indian Red Jungle Fowl (Gallus gallus murghi) Semen

机译：冷冻保存对脂质过氧化，抗氧化潜力，染色质完整性，印度红丛林禽（巨大Gallus Murghi）精液的抗氧化势，染色质完整性和线粒体活性

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Aim: The study elucidates the impact of cryopreservation on lipid peroxidation (LPO), antioxidant potential, DNA integrity, and mitochondrial activity of Indian red jungle fowl sperm. Materials and Methods: Semen from eight mature cocks was pooled and diluted at 37 degrees C using a specific medium for red fowl species. The diluted semen samples were immediately refrigerated at 4 degrees C for 2 hours (0.275 degrees C min(-1)). Glycerol was then added to a final concentration of 20%, and the samples were equilibrated for 10 minutes at 5 degrees C before loading into 0.25 mL French straws. These straws were then frozen by placing them in liquid nitrogen vapor for 10 minutes and plunged into liquid nitrogen. Motility, viability, DNA integrity, antioxidant activity, and LPO were assessed before dilution (fresh semen), after equilibration (processed semen), and post-thawing (frozen semen). Results: Sperm motility, viability, DNA integrity, and mitochondrial activity decreased (p < 0.05) in processed and frozen semen compared with fresh semen. Nevertheless, the concentration of malondialdehyde (MDA) in sperm and seminal plasma was greater (p < 0.05) in frozen-thawed and processed semen compared with fresh semen. Multivariate regression analysis showed a negative impact of MDA concentration in sperm (R-2 = 0.90, Wilk's lambda = 0.003, p < 0.001) and seminal plasma (R-2 = 0.84, Wilk's lambda = 0.02, p < 0.001) on motility, viability, DNA integrity, and mitochondrial activity. Nonetheless, total antioxidant capacity (TAC) had a positive impact on sperm variables (R-2 = 0.82, Wilk's lambda = 0.096, p < 0.001). Conclusions: The decrease in motility, viability, DNA integrity, and mitochondrial activity of Indian red jungle fowl sperm was associated with an increase in LPO during cryopreservation. Furthermore, TAC was reduced during the freeze-thaw process, which was insufficient in protecting the sperm against high reactive oxygen species levels.
机译：目的：该研究阐明了光束对脂质过氧化（LPO），抗氧化势，DNA完整性和线粒体活性的影响。材料和方法：使用八个成熟的鸡尾酸的精液使用37摄氏度稀释37摄氏度。将稀释的精液样品在4℃下冷冻2小时（0.275℃min（-1））。然后将甘油加入到终浓度为20％的浓度下，并在装入0.25ml法国吸管之前将样品在5℃下在5℃下平衡10分钟。然后通过将这些吸管在液氮蒸汽中放入10分钟并填充到液氮中来冷冻。在稀释（新鲜精液），在平衡（加工精液）和后解冻（冷冻精液）之前评估运动，活力，DNA完整性，抗氧化活性和LPO。结果：与新鲜精液相比，加工和冷冻精液的精子运动，活力，DNA完整性和线粒体活性降低（P <0.05）。然而，与新鲜精液相比，冷冻解冻和加工精液的精子和精液中丙二醛（MDA）的浓度更大（P <0.05）。多元回归分析显示MDA浓度在精子中的负面影响（R-2 = 0.90，Wilk的Lambda = 0.003，P <0.001）和精体血浆（R-2 = 0.84，Wilk的Lambda = 0.02，P <0.001），活力，DNA完整性和线粒体活性。尽管如此，总抗氧化能力（TAC）对精子变量具有正影响（R-2 = 0.82，Wilk的Lambda = 0.096，P <0.001）。结论：印度红丛林禽精子的运动性，活力，DNA完整性和线粒体活性的降低与冷冻保存期间的LPO增加有关。此外，在冻融过程中，TAC降低，这在保护精子不足以保护精子免受高反应性氧物质水平。

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