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首页> 外文期刊>Biopreservation and biobanking >Addition of Equex STM to Extender Improves Post-Thawing Longevity of Collared Peccaries' Sperm
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Addition of Equex STM to Extender Improves Post-Thawing Longevity of Collared Peccaries' Sperm

机译：添加了STM的Extender改善了雄性剖宫产的后期寿命

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摘要

The effect of Equex STM (R) paste supplementation on the Tris-extender for collared peccaries' semen cryopreservation was assessed. Semen from 12 mature individuals was obtained by electroejaculation and evaluated for morphology, membrane integrity, osmotic response, and sperm kinetic metrics. Samples were diluted in Tris plus 20% egg yolk and divided into three aliquots. The first aliquot was without any supplementation, the second and third contained 0.5 and 1.0% Equex STM, respectively. The samples were added with 3% glycerol, frozen in liquid nitrogen, thawed, and assessed for the same parameters after a thermal resistance test (TRT) for 120 minutes. Similar values were detected for the different treatments immediately after thawing, except for the amplitude lateral head that was reduced in samples containing Equex (p < 0.05). During TRT, samples containing Equex were more efficient in preserving the sperm motility (at 0.5%: 25.5% +/- 4.4%; at 1%: 33.3% +/- 6.3%) at 30 minutes, in comparison with the control group (16.6% +/- 6.0%), in which sperm motility decreased at 15 minutes (p < 0.05). Moreover, Equex, especially at 0.5% concentration, was able to maintain plasma membrane integrity and sperm motility in all the samples after incubation for 60 minutes. In conclusion, we recommend the addition of Equex STM at 0.5% to the Tris-extender to improve post-thawing sperm longevity in collared peccaries.
机译：评估了STM（R）粘贴对雄性剖腹产干燥的Tris-extender的效果的影响。通过ElectroeRacucation获得来自12个成熟个体的精液，并评估形态，膜完整性，渗透反应和精子动力学度量。将样品在Tris加20％蛋黄中稀释，并分为三个等分试样。第一种等分试样在没有任何补充，第二和第三等分试样分别含有0.5和1.0％的STM。将样品加入3％甘油，冷冻在液氮中冷冻，解冻，并在热阻试验（TRT）之后评估相同的参数120分钟。除了在含有SECEX的样品中减少的幅度横向头，除了在解冻之后立即检测到类似的值（P <0.05）。在TRT期间，含有EQSEX的样品在保留精子运动方面更有效（0.5％：25.5％+/- 4.4％;与对照组相比，在30分钟后在30分钟内为1％：33.3％+/- 6.3％）（ 16.6％+/- 6.0％），其中精子活力在15分钟内降低（P <0.05）。此外，Equax，特别是0.5％浓度，在孵育后，能够在所有样品中维持血浆膜完整性和精子活力60分钟。总之，我们建议向Tris-Extender添加0.5％的STM，以改善锁上的Peccaries中的解冻精子寿命。

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  • 来源
    《Biopreservation and biobanking》 |2019年第2期|共5页
  • 作者

    Bezerra Luana G. P.; Souza Ana L. P.; Lago Arthur E. A.; Campos Livia B.; Nunes Talita L.; Paula Valeria V.; Oliveira Moacir F.; Silva Alexandre R.;

  • 作者单位

    Univ Fed Rural Semiarido UFERSA Lab Anim Germplasm Conservat LCGA Dept Anim Sci Ctr Ciencias Agr Francisco Mota Ave 572 BR-59700000 Mossoro RN Brazil;

    Univ Fed Rural Semiarido UFERSA Lab Anim Germplasm Conservat LCGA Dept Anim Sci Ctr Ciencias Agr Francisco Mota Ave 572 BR-59700000 Mossoro RN Brazil;

    Univ Fed Rural Semiarido UFERSA Lab Anim Germplasm Conservat LCGA Dept Anim Sci Ctr Ciencias Agr Francisco Mota Ave 572 BR-59700000 Mossoro RN Brazil;

    Univ Fed Rural Semiarido UFERSA Lab Anim Germplasm Conservat LCGA Dept Anim Sci Ctr Ciencias Agr Francisco Mota Ave 572 BR-59700000 Mossoro RN Brazil;

    Univ Fed Rural Semiarido UFERSA Lab Anim Germplasm Conservat LCGA Dept Anim Sci Ctr Ciencias Agr Francisco Mota Ave 572 BR-59700000 Mossoro RN Brazil;

    Univ Fed Rural Semiarido UFERSA Lab Anim Germplasm Conservat LCGA Dept Anim Sci Ctr Ciencias Agr Francisco Mota Ave 572 BR-59700000 Mossoro RN Brazil;

    Univ Fed Rural Semiarido UFERSA Lab Anim Germplasm Conservat LCGA Dept Anim Sci Ctr Ciencias Agr Francisco Mota Ave 572 BR-59700000 Mossoro RN Brazil;

    Univ Fed Rural Semiarido UFERSA Lab Anim Germplasm Conservat LCGA Dept Anim Sci Ctr Ciencias Agr Francisco Mota Ave 572 BR-59700000 Mossoro RN Brazil;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Tayassu tajacu; semen; wildlife; biobank; SDS;

    机译：Tayassu Tajacu;精液;野生动物;Biobank;SDS;

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