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Effects of Tween 80 on fermentative butanol production from alkali-pretreated switchgrass

机译：吐温80对碱预处理开关的发酵丁醇产量的影响

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In this study, switchgrass was investigated as a renewable feedstock for fermentative production of acetone, butanol and ethanol (ABE). For this purpose, switchgrass was first subjected to alkaline pretreatment, which reduces its recalcitrance and increases enzyme accessibility. Both simultaneous saccharification and fermentation (SSF) and separate hydrolysis and fermentation (SHF) were applied. The pretreated switchgrass solids inhibit ABE fermentation, presumably due to the residual lignin portion, as evidenced by the toxicity test of various model phenolic compounds. Addition of a non-ionic surfactant, Tween 80, alleviated the phenol-induced inhibition. As constrained by the "solid effects", the upper limit of solid loading that SSF could process was in the range of 5-6%. SSF with 5% solid loading produced a total of 12.3 ABE solvents with 7.8 g/L of butanol. SHF was further applied to process higher solid loadings. A total of 14.3 g/L. ABE solvent with 8.5 g/L of butanol was produced from SHF with 7% solid loading. SSF gave an overall bioconversion yield close to that of pure sugar control, whereas SHF was able to avoid the "solid effects" with a slightly lower overall bioconversion yield. (C) 2018 Elsevier B.V. All rights reserved.
机译：在这项研究中，研究了切换紫杉，作为可再生原料的丙酮，丁醇和乙醇（ABE）的发酵生产。为此目的，首先对碱性预处理进行切换，这降低了其核批分并增加了酶可用性。施加同时糖化和发酵（SSF）和单独的水解和发酵（SHF）。预处理的交换机固体抑制ABE发酵，可能是由于残留的木质素部分，如各种模型酚类化合物的毒性试验所证明。添加非离子表面活性剂，吐温80，减轻了酚诱导的抑制作用。受到“固体效应”的约束，SSF可以加工的固体负载的上限在5-6％的范围内。具有5％固体载荷的SSF共产生12.3 ABE溶剂，丁醇的丁醇7.8g / L. SHF进一步应用于加工更高的固体载荷。共14.3克/升。具有8.5g / L丁醇的ABE溶剂由SHF制备，具有7％固体载荷。 SSF对纯糖控制的整体生物转化率较好，而SHF能够避免“固体效应”，略低于整体生物转化产量。 （c）2018 Elsevier B.v.保留所有权利。

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