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首页> 外文期刊>Bioscience, Biotechnology, and Biochemistry >Characterization of a yam class IV chitinase produced by recombinant Pichia pastoris X-33
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Characterization of a yam class IV chitinase produced by recombinant Pichia pastoris X-33

机译：通过重组Pichia Pastoris X-33产生的山药IV丁蛋白酶的表征

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摘要

A yam (Dioscorea opposita Thunb) class IV chitinase, whose genomic DNA was cloned by Mitsunaga et al. (2004), was produced by the recombinant Pichia pastoris X-33 in high yields such as 66 mg/L of culture medium. The chitinase was purified by column chromatography after Endoglycosidase H treatment and then characterized. It showed properties similar to the original chitinase E purified from the yam tuber reported by Arakane et al. (2000). This Pichia-produced chitinase also showed strong lytic activity against Fusarium oxysporum and Phytophthora nicotianae, wide pH and thermal stability, optimum activity at higher temperature such as 70 degrees C, and high substrate affinity, indicating that one can use this Pichia-produced yam chitinase as a bio-control agent.
机译：山药（Dioscorea Opposita Thunb）四类胰蛋白酶，其基因组DNA被Mitsunaga等人克隆。 （2004），由重组Pichia Pastoris X-33生产高产率，例如66mg / L培养基。 通过柱色谱法进行柱色谱纯化几丁质酶，然后进行表征。 它显示出类似于由Arakane等人报告的山药块茎纯化的原始胰蛋白酶E. （2000）。 本次碱生产的章节酶还表现出对镰刀菌和植物孢子菌和植物的强烈裂解活性，宽的pH和热稳定性，在较高温度下的最佳活性，如70℃，高底物亲和力，表明可以使用该Pichia制造的山氨基丁蛋白酶 作为生物对照剂。

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