...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cytokine >The differential effect of Eriobotrya japonica hydrophilic leaf extract on cytokines production and modulation.
【24h】

The differential effect of Eriobotrya japonica hydrophilic leaf extract on cytokines production and modulation.

机译：中华绒螯蟹亲水性叶提取物对细胞因子产生和调节的差异作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Stimulating or modulating the release of cytokines by immunomodulators or immunostimulating agents is an attractive mode for treating several diseases such as viral infections. For instance, patients with viral infections may be in need of increasing or inducing T helper 1 (Th1) or proinflammatory cytokines, which ultimately activate T cytotoxic and Natural killer lymphocytes to kill virally infected cells. Of these agents, we found that Eriobotrya japonica hydrophilic leaf extract (EJHE) can induce and modulate cytokines in dose-dependent manner. Twenty-four hour exposure of increasing concentrations of EJHE increased significantly (p<0.001) the production of IFN-gamma and TNF-alpha, from PHA+LPS-stimulated whole blood. However, the production of IFN-gamma and TNF-alpha plateaued at high EJHE concentrations (10-100mug/ml). No significant changes in the production of IL-10 were seen. In addition, EJHE at 1 and 10mug/ml reversed significantly (p<0.01) the inhibitory effect of hydrocortisone on the IL-12p70, IFN-gamma and TNF-alpha production from PHAS+LPS stimulated whole blood. Without PHA and LPS, EJHE was found to induce significantly (p<0.001) IFN-gamma, IL-12 p70, TNF-alpha, and IL-10 from whole blood culture in concentration dependent manner. The maximum induction of IFN-gamma, IL-12 p70, and TNF-alpha by EJHE was at 1 and 10mug/ml. On the other hand, IL-10 induction kept increasing even at the highest concentration used (100mug/ml) of EJHE. Furthermore, intra-peritoneal injection of EJHE into mice increased significantly serum cytokines level mainly at 10 and 100mug/ml. Two-hour post i.p. injection, EJHE increased serum IFN-gamma, TNF-alpha, and IL-10 to approximately 750, 1000, and 250pg/ml, respectively. However, 24h post i.p. injection, the levels of TNF-alpha, and IL-10 were similar to basal levels but IFN-gamma levels were 200pg/ml. These results indicate that EJHE induces proinflammatory and Th1 cytokines in concentration dependent manner and the effect of this induction should be studied further in viral models to check the efficacy of such cytokine induction.
机译：通过免疫调节剂或免疫刺激剂刺激或调节细胞因子的释放是治疗几种疾病如病毒感染的有吸引力的方式。例如，病毒感染患者可能需要增加或诱导T辅助1（Th1）或促炎性细胞因子，从而最终激活T细胞毒性和天然杀伤性淋巴细胞杀死病毒感染的细胞。在这些药物中，我们发现日本绒肠亲水叶提取物（EJHE）可以剂量依赖的方式诱导和调节细胞因子。从PHA + LPS刺激的全血中，增加浓度的EJHE暴露二十四小时会显着增加（p <0.001）IFN-γ和TNF-α的产生。但是，在高EJHE浓度（10-100μg/ ml）时，IFN-γ和TNF-α的生成稳定。没有观察到IL-10产生的显着变化。此外，以1和10 ug / ml的EJHE显着逆转（p <0.01）氢化可的松对PHAS + LPS刺激的全血IL-12p70，IFN-γ和TNF-α产生的抑制作用。在没有PHA和LPS的情况下，发现EJHE以浓度依赖性方式从全血培养物中明显诱导（p <0.001）IFN-γ，IL-12 p70，TNF-α和IL-10。 EJHE对IFN-γ，IL-12 p70和TNF-α的最大诱导分别为1和10mug / ml。另一方面，即使在最高浓度的EJHE（100mug / ml）中，IL-10诱导也保持增加。此外，向小鼠腹膜内注射EJHE可以显着增加血清细胞因子水平，主要在10和100μg/ ml。 i.p.两小时后注射后，EJHE将血清IFN-γ，TNF-α和IL-10分别提高至大约750、1000和250pg / ml。但是，i.p。后24小时注射时，TNF-α和IL-10的水平与基础水平相似，但IFN-γ的水平为200pg / ml。这些结果表明，EJHE以浓度依赖的方式诱导促炎和Th1细胞因子，这种诱导的作用应在病毒模型中进一步研究，以检查这种细胞因子诱导的功效。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号