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首页> 外文期刊>Cytokine >Induction of Th1 cytokines by Leuconostoc mesenteroides subsp. mesenteroides (KCTC 3100) under Th2-type conditions and the requirement of NF-kappaB and p38/JNK.
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Induction of Th1 cytokines by Leuconostoc mesenteroides subsp. mesenteroides (KCTC 3100) under Th2-type conditions and the requirement of NF-kappaB and p38/JNK.

机译：mesenteroides亚种诱导Th1细胞因子。在Th2型条件下以及要求NF-kappaB和p38 / JNK的情况下，肠膜膜肠炎（KCTC 3100）。

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摘要

Leuconostoc mesenteroides subsp. mesenteroides (LMM) KCTC 3100, is one of the prominent species in the fermentation of kimchi, a traditional Korean food. In the present study, we investigated the capacity of this microorganism in inducing Th1 cytokines in the presence of Th2 signals in vitro and in vivo and the requirement of NF-kappaB and MAPK signaling. Stimulation with heat-killed LMM in mouse splenocytes induced the expression of IFN-gamma, which was dependent on IL-12 production by LMM. Pre-treatment with LMM in vitro augmented the production of IFN-gamma and IL-4 in response to anti-CD3 plus recombinant IL-4 (rIL-4). LMM administration to mice, beginning either before or after the development of OVA sensitization, increased OVA-restimulated IFN-gamma production in the splenocytes and reduced serum total and OVA-specific IgE levels. However, only the pre-sensitization treatment induced a slight reduction in IL-4 from the same cells, but the post-sensitization treatment did not. Induction of IL-12 by LMM in peritoneal macrophages involved NF-kappaB, p38 and JNK, but not ERK1/2. In conclusion, our data presented the upregulation of IFN-gamma by LMM under the pro-Th2 conditions and the requirement of NF-kappaB, p38 and JNK for IL-12 production. These observations suggest that this microorganism can be a useful Th1-inducing agent in modulating the Th1/Th2 imbalance.
机译：mesenteroides亚种。 mesenteroides（LMM）KCTC 3100是韩国传统食品泡菜发酵中的重要物种之一。在本研究中，我们调查了这种微生物在体外和体内存在Th2信号的情况下诱导Th1细胞因子的能力以及对NF-κB和MAPK信号的需求。在小鼠脾细胞中用热杀死的LMM刺激诱导IFN-γ的表达，这依赖于LMM产生的IL-12。体外用LMM预处理可增加抗-CD3加重组IL-4（rIL-4）的IFN-γ和IL-4的产生。在发生OVA致敏之前或之后开始对小鼠进行LMM给药，增加了脾细胞中OVA重新刺激的IFN-γ产生，并降低了血清总和OVA特异性IgE水平。然而，仅敏化前处理诱导来自相同细胞的IL-4略微降低，但是敏化后处理未诱导。 LMM在腹膜巨噬细胞中诱导IL-12涉及NF-κB，p38和JNK，但不涉及ERK1 / 2。总之，我们的数据显示了在Th2亲原条件下LMM对IFN-γ的上调，以及IL-12产生对NF-κB，p38和JNK的需求。这些观察结果表明，这种微生物可以在调节Th1 / Th2失衡中成为有用的Th1诱导剂。

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