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首页> 外文期刊>Cytokine >Cloning, expression analysis and bioactivity studies of rainbow trout (Oncorhynchus mykiss) interleukin-22.
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Cloning, expression analysis and bioactivity studies of rainbow trout (Oncorhynchus mykiss) interleukin-22.

机译：虹鳟鱼白介素22的克隆，表达分析和生物活性研究。

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摘要

This report describes the cloning and characterisation of rainbow trout (Oncorhynchus mykiss) interleukin (IL)-22, and presents studies of the functional activity of its recombinant protein for the first time in a non-mammalian species. The predicted IL-22 coding region consists of 522 nucleotides which translates into a 173 amino acid protein, that contains an IL-10 family signature which is reasonably well conserved with other vertebrate IL-22 molecules. Expression analysis in tissues from healthy fish revealed a higher constitutive expression of IL-22 in mucosal tissues, suggesting a potentially important role in mucosal immunity. In vitro studies demonstrated that IL-22 expression was induced significantly by PHA and PMA in splenocyte primary cultures 4h post-stimulation. Expression was also induced in the spleen upon infection of fish with the Gram-negative bacterium Yersinia ruckeri, suggesting a potential role of IL-22 in vivo in defence against bacterial diseases. The Escherichia coli produced recombinant IL-22 enhanced the expression of a number of antimicrobial peptides, promoting host innate immunity against microbes and revealing a biological similarity with its mammalian counterpart.
机译：该报告描述了虹鳟（Oncorhynchus mykiss）白介素（IL）-22的克隆和特征，并首次提出了其重组蛋白在非哺乳动物物种中的功能活性研究。预测的IL-22编码区由522个核苷酸组成，可翻译为173个氨基酸的蛋白质，其中包含IL-10家族标记，与其他脊椎动物IL-22分子相当保守。在健康鱼类组织中的表达分析表明，粘膜组织中IL-22的组成型表达较高，提示其在粘膜免疫中的潜在重要作用。体外研究表明，刺激后4h，PHA和PMA在脾细胞原代培养物中显着诱导IL-22表达。在鱼中被革兰氏阴性细菌Rersinia ruckeri感染后，也会在脾脏中诱导表达，表明IL-22在体内对细菌性疾病的防御中具有潜在作用。大肠杆菌产生的重组IL-22增强了许多抗菌肽的表达，促进了宿主对微生物的先天免疫力，并显示了与哺乳动物对应物的生物学相似性。

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