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首页> 外文期刊>Cytokine >L929 cell conditioned medium protects RAW264.7 cells from oxidative injury through inducing antioxidant enzymes.
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L929 cell conditioned medium protects RAW264.7 cells from oxidative injury through inducing antioxidant enzymes.

机译:L929细胞条件培养基可通过诱导抗氧化酶来保护RAW264.7细胞免受氧化损伤。

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摘要

We have previously found that L929 cell conditioned medium (L929-CM) could protect mouse peritoneal macrophages from oxidative injury. To uncover the mechanism further, we investigated the effect of L929-CM on the oxidative injury caused by tbOOH to RAW264.7 cell lines. The results showed that L929-CM could protect RAW264.7 cells from oxidative injury (presented by cell morphology and cell survival rate), and L929-CM could also improve total superoxide dismutase (SOD), selenium-dependent and non-selenium-dependent glutathione peroxidase (SeGPx and non-SeGPx) activities in RAW264.7 cells. RT-PCR analysis showed that, L929-CM could induce plasma glutathione peroxidase (PLGPx) mRNA expression, while there was no inducing effect of L929-CM on phospholipid hydroperoxide glutathione peroxidase (PHGPx) mRNA expression in RAW264.7 cells. 5 &mgr;g/ml actinomycin D, 30 microg/ml cycloheximide (de novo protein synthesis inhibitor) and 50 microg/ml acetovanilone (intracellular superoxide anion production inhibitor) had no effects in attenuating the induction of PLGPx expression by L929-CM. Copyright 2000 Academic Press.
机译:我们以前已经发现,L929细胞条件培养基(L929-CM)可以保护小鼠腹膜巨噬细胞免受氧化损伤。为了进一步揭示该机制,我们研究了L929-CM对tbOOH引起的RAW264.7细胞系氧化损伤的影响。结果表明,L929-CM可以保护RAW264.7细胞免受氧化损伤(由细胞形态和细胞存活率表示),并且L929-CM还可以改善总超氧化物歧化酶(SOD),硒依赖性和非硒依赖性RAW264.7细胞中的谷胱甘肽过氧化物酶(SeGPx和非SeGPx)活性。 RT-PCR分析表明,L929-CM可以诱导血浆谷胱甘肽过氧化物酶(PLGPx)mRNA表达,而L929-CM对RAW264.7细胞中的磷脂氢过氧化物谷胱甘肽过氧化物酶(PHGPx)mRNA表达没有诱导作用。 5μg/ ml放线菌素D,30μg/ ml环己酰亚胺(从头蛋白合成抑制剂)和50μg/ ml乙酰丙酮(细胞内超氧阴离子产生抑制剂)对减弱L929-CM对PLGPx表达的诱导没有影响。版权所有2000学术出版社。

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