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首页> 外文期刊>Cytokine >Cytokine and free radical responses of alveolar macrophages in vitro to asbestos fibres.
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Cytokine and free radical responses of alveolar macrophages in vitro to asbestos fibres.

机译:体外肺泡巨噬细胞对石棉纤维的细胞因子和自由基反应。

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摘要

A method for culturing primary rat alveolar macrophages (AMs) for 14 days was used to compare their responses to crocidolite and chrysotile asbestos fibres. Exposure to crocidolite increased production of tumour necrosis factor-alpha (TNF-alpha) and interleukin 1beta (IL-1beta), whereas exposure to chrysotile did not; neither fibre altered the production of interleukin 6 (IL-6). IL-1beta production increased progressively, while TNF-alpha was fully elevated from day 1. Conversely, chrysotile, but not crocidolite, increased production of superoxide anion and nitric oxide (NO) radicals. These differential responses were only observed by extending the culture beyond the usual 1-3 days. Copyright 2000 Academic Press.
机译:一种用于培养大鼠原发性肺泡巨噬细胞(AM)14天的方法用于比较它们对青石棉和温石棉石棉纤维的反应。暴露于青石棉会增加肿瘤坏死因子-α(TNF-alpha)和白介素1beta(IL-1beta)的产生,而接触温石棉则不会;两种纤维均未改变白介素6(IL-6)的产生。从第1天开始,IL-1β的产生逐渐增加,而TNF-α则完全升高。相反,温石棉而不是青石棉增加了超氧阴离子和一氧化氮(NO)自由基的产生。仅通过将培养物延长至通常的1-3天才能观察到这些差异反应。版权所有2000学术出版社。

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