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首页> 外文期刊>Cytokine >Effects of sample handling on the stability of interleukin 6, tumour necrosis factor-alpha and leptin.
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Effects of sample handling on the stability of interleukin 6, tumour necrosis factor-alpha and leptin.

机译：样品处理对白介素6，肿瘤坏死因子-α和瘦素稳定性的影响。

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摘要

Detected levels of IL-6, TNF-alpha and leptin may be affected by methods of storage, anticoagulant or repeated freezing-thawing. Blood samples from 22 healthy subjects were: (i) allowed to stand for 1, 2, 4 or 6 h prior to, or after separation, before freezing at -70 degrees C; (ii) taken into tubes with lithium heparin, sodium citrate, EDTA or no anticoagulant, separated and frozen; and (iii) separated, and plasma repeatedly freeze-thawed for up to six cycles prior to assay. Leptin was assayed by RIA, and IL-6 and TNF-alpha by high-sensitivity ELISA. (i) IL-6 and TNF-alpha levels were not altered significantly in separated samples but IL-6 declined by mean (SEM) 14.3% (3.7%) and TNF-alphaincreased by 9.6% (2.3%) in samples left unseparated for 4 h (P=0.003 and 0.002, respectively). Leptin remained unchanged. (ii) Serum and EDTA-plasma samples gave comparable results for all three cytokines, but levels in the other anticoagulant samples were highly variable. (iii) IL-6 and leptin levels were not altered by up to 6 cycles of freeze-thawing, but TNF-alpha increased by 17.0% (3.7%) after 3 cycles. Concentrations of these molecules are significantly altered by storage conditions, therefore they need to be standardized for epidemiological and clinical studies, and between-study comparisons of levels may not be reliable. Copyright 2000 Academic Press.
机译：检测到的IL-6，TNF-α和瘦素水平可能受储存，抗凝或反复冻融方法的影响。来自22位健康受试者的血样：（i）在分离之前或之后静置1、2、4或6 h，然后在-70摄氏度冷冻。 （ii）放入装有肝素锂，柠檬酸钠，EDTA或无抗凝剂的试管中，分离并冷冻; （iii）分离，在测定前将血浆反复冻融多达六个循环。瘦素通过RIA测定，IL-6和TNF-α通过高灵敏度ELISA测定。 （i）分离样品中的IL-6和TNF-α水平没有明显改变，但未分离样品中的IL-6平均值（SEM）下降了14.3％（3.7％），TNF-α上升了9.6％（2.3％）。 4小时（分别为P = 0.003和0.002）。瘦素保持不变。 （ii）血清和EDTA血浆样品对所有三种细胞因子均具有可比的结果，但其他抗凝剂样品中的水平却高度可变。 （iii）最多6个周期的冻融循环不会改变IL-6和瘦素的水平，但3个周期后TNF-α却增加了17.0％（3.7％）。这些分子的浓度会因储存条件而显着改变，因此需要对它们进行流行病学和临床研究的标准化，并且研究之间的水平比较可能不可靠。版权所有2000学术出版社。

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