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首页> 外文期刊>Cytokine >Th1/Th2 Cytometric Bead Array can discriminate cytokine secretion from endogenously activated cells in pulmonary disease, recent and remote infection in tuberculosis.
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Th1/Th2 Cytometric Bead Array can discriminate cytokine secretion from endogenously activated cells in pulmonary disease, recent and remote infection in tuberculosis.

机译：Th1 / Th2细胞珠阵列可以区分肺部疾病，结核病的近期和远处感染中内源性激活细胞的细胞因子分泌。

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摘要

Differential T cell trafficking through the blood compartment towards infected foci may be occurring in different stages of tuberculosis disease and infection. The aim of the present study was to identify cytokine signatures in the blood compartment in tuberculosis patients with pulmonary disease (PTB=19), recently exposed household contacts (HC=27) and nonexposed community controls (EC=37). Diluted (1:10) whole blood was cultured for 2 days and cytokine secretion was assessed using Cytometric Bead Array (Th1/Th2 kit II; BD Biosciences) which included IL-2, TNF-alpha, IFN-gamma (Type1/T1), IL-4, IL-6 and IL-10 (Type2/T2). All T1/T2 cytokines were elevated in PTB (AUROC>0.9) while HC showed selective elevation of IL-6 (AUROC>0.7) compared to EC. Principal component analysis (PCA) extracted two groupings with Eigen values >1; IL-6 separated into the second component for PTB, HC and EC. After rotation, IFN-gamma was correlated with the first component for PTB and EC and the second component for HC indicating an absence of T1/T2 dichotomy. Therefore endogenous cytokine signatures may indicate differential T cell trafficking in different stages of tuberculosis infection and disease.
机译：在结核病和感染的不同阶段，可能会发生通过血液腔室向感染灶的差异性T细胞运输。本研究的目的是确定患有肺部疾病（PTB = 19），最近接触过家庭接触者（HC = 27）和未接触社区控制者（EC = 37）的结核病患者血液腔室中的细胞因子特征。将稀释的（1:10）全血培养2天，并使用Cytometric Bead Array（Th1 / Th2试剂盒II; BD Biosciences）评估细胞因子的分泌，其中包括IL-2，TNF-α，IFN-γ（Type1 / T1） ，IL-4，IL-6和IL-10（Type2 / T2）。与EC相比，PTB中所有T1 / T2细胞因子均升高（AUROC> 0.9），而HC显示IL-6选择性升高（AUROC> 0.7）。主成分分析（PCA）提取了两个特征值> 1的分组。 IL-6分为PTB，HC和EC的第二部分。旋转后，IFN-γ与PTB和EC的第一成分以及HC的第二成分相关，表明不存在T1 / T2二分法。因此，内源性细胞因子信号可能表明在结核病感染和疾病的不同阶段，差异性的T细胞运输。

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