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首页> 外文期刊>Cytokine >IL-10 and IFN-γ gene expression in chronic Chagas disease patients after in vitro stimulation with recombinant antigens of Trypanosoma cruzi
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IL-10 and IFN-γ gene expression in chronic Chagas disease patients after in vitro stimulation with recombinant antigens of Trypanosoma cruzi

机译：克鲁格锥虫重组抗原体外刺激后慢性查加斯病患者IL-10和IFN-γ基因表达

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Along with several other aspects of Chagas disease, the mechanisms responsible for the different clinical outcomes observed in chronic infected individuals have not yet been clarified. It is believed that the host immune response to the parasite plays an important role in the development of the pathology. Therefore, the aim of this study was to evaluate the relationship between IL-10 and IFN-γ gene expression profile, after in vitro stimulation of peripheral blood mononuclear cells (PBMC) with Trypanosoma cruzi recombinant antigens CRA (cytoplasmatic repetitive antigen) and FRA (flagellar repetitive antigen), and the clinical forms of chronic Chagas disease. Twenty patients with the cardiac form of the disease (CARD), of whom 10 had the mild cardiac form (CARD 1) and 10 the severe cardiac form (CARD 2), and 20 patients with the indeterminate form (IND), were selected at the Chagas Disease Unit of the Oswaldo Cruz University Hospital, University of Pernambuco, Recife, Pernambuco, Brazil. The PBMCs of these individuals were cultured in the presence of CRA or FRA for 3. days and IL-10 and IFN-γ gene expression was evaluated by detection of its messenger RNA using Real Time Quantitative PCR. Although no significant difference was observed between the groups of individuals studied, we found that most patients with IND displayed high levels of IFN-γ gene expression, while the majority of patients with CARD 1 presented high levels of IL-10. The results of this study thus highlight the important role that inflammatory cytokines play in patients with the IND group controlling for parasite replication, and that anti-inflammatory cytokines play in determining susceptibility to progression to symptomatic clinical forms of the disease.
机译：除了查加斯病（Chagas disease）的其他几个方面外，尚不清楚造成慢性感染个体中不同临床结果的机制。相信宿主对寄生虫的免疫反应在病理学发展中起重要作用。因此，本研究的目的是评估在用克鲁氏锥虫重组抗原CRA（胞质重复抗原）和FRA体外刺激外周血单个核细胞（PBMC）后，IL-10和IFN-γ基因表达谱之间的关系。鞭毛重复抗原）和慢性恰加斯病的临床形式。选择20例具有心脏疾病形式（CARD）的患者，其中10例患有轻度心脏形式（CARD 1），10例具有严重心脏形式（CARD 2），以及20例不确定形式（IND）。巴西伯南布哥累西腓伯南布哥大学奥斯瓦尔多·克鲁兹大学医院恰加斯病科。在CRA或FRA存在下将这些个体的PBMC培养3天，并通过使用实时定量PCR通过检测其信使RNA来评估IL-10和IFN-γ基因表达。尽管在研究的个体组之间未观察到显着差异，但我们发现大多数IND患者表现出高水平的IFN-γ基因表达，而大多数CARD 1患者表现出高水平的IL-10。因此，这项研究的结果强调了炎症细胞因子在控制寄生虫复制的IND组患者中的重要作用，以及抗炎细胞因子在确定疾病发展为症状性临床形式的易感性中的重要作用。

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