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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Expression, crystallization and preliminary X-ray crystallographic analysis of cellobiose 2-epimerase from Dictyoglomus turgidum DSM 6724
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Expression, crystallization and preliminary X-ray crystallographic analysis of cellobiose 2-epimerase from Dictyoglomus turgidum DSM 6724

机译：杜氏梭菌DSM 6724纤维二糖2-表异构酶的表达，结晶及初步X射线晶体学分析

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摘要

Cellobiose 2-epimerase epimerizes and isomerizes beta-1,4- and alpha-1,4-gluco-oligosaccharides. N-Acyl-D-glucosamine 2-epimerase (DT_epimerase) from Dictyoglomus turgidum has an unusually high catalytic activity towards its substrate cellobiose. DT_epimerase was expressed, purified and crystallized. Crystals were obtained of both His-tagged DT_epimerase and untagged DT_epimerase. The crystals of His-tagged DT_epimerase diffracted to 2.6 angstrom resolution and belonged to the monoclinic space group P2(1), with unit-cell parameters a = 63.9, b = 85.1, c = 79.8 angstrom, beta = 110.8 degrees. With a Matthews coefficient V-M of 2.18 angstrom(3) Da(-1), two protomers were expected to be present in the asymmetric unit with a solvent content of 43.74%. The crystals of untagged DT_epimerase diffracted to 1.85 angstrom resolution and belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 55.9, b = 80.0, c = 93.7 angstrom. One protomer in the asymmetric unit was expected, with a corresponding V-M of 2.26 angstrom(3) Da(-1) and a solvent content of 45.6%.
机译：纤维二糖2-表异构酶差向异构化β-1,4-和α-1,4-葡萄糖寡糖。来自杜鹃小球藻的N-酰基-D-葡糖胺2-表异构酶（DT_epimerase）对其底物纤维二糖具有异常高的催化活性。 DT_epimerase被表达，纯化和结晶。获得了带有His标签的DT_epimerase和未标签的DT_epimerase的晶体。带有His标签的DT_epimerase晶体衍射至2.6埃分辨率，属于单斜晶空间群P2（1），其晶胞参数a = 63.9，b = 85.1，c = 79.8埃，β= 110.8度。具有2.18埃（3）Da（-1）的Matthews系数V-M，预期在不对称单元中存在两个protomer，溶剂含量为43.74％。未标记的DT_epimerase晶体衍射至1.85埃分辨率，属于正交晶空间群P2（1）2（1）2（1），单位晶胞参数a = 55.9，b = 80.0，c = 93.7埃。预期在不对称单元中有一个准分子，相应的V-M为2.26埃（3）Da（-1），溶剂含量为45.6％。

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