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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser
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Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser

机译：使用X射线自由电子激光在环境温度下悬浮液中30S核糖体亚基微晶的系列飞秒X射线衍射

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摘要

High-resolution ribosome structures determined by X-ray crystallography have provided important insights into the mechanism of translation. Such studies have thus far relied on large ribosome crystals kept at cryogenic temperatures to reduce radiation damage. Here, the application of serial femtosecond X-ray crystallography (SFX) using an X-ray free-electron laser (XFEL) to obtain diffraction data from ribosome microcrystals in liquid suspension at ambient temperature is described. 30S ribosomal subunit microcrystals diffracted to beyond 6 angstrom resolution, demonstrating the feasibility of using SFX for ribosome structural studies. The ability to collect diffraction data at near-physiological temperatures promises to provide fundamental insights into the structural dynamics of the ribosome and its functional complexes.
机译：通过X射线晶体学确定的高分辨率核糖体结构已为翻译机制提供了重要见识。迄今为止，此类研究依赖于保持在低温下的大核糖体晶体以减少辐射损伤。在此，描述了使用X射线自由电子激光器（XFEL）的连续飞秒X射线晶体学（SFX）从环境温度下的液体悬浮液中的核糖体微晶获得衍射数据的应用。 30S核糖体亚基微晶衍射到超过6埃的分辨率，证明了使用SFX进行核糖体结构研究的可行性。在接近生理温度的温度下收集衍射数据的能力有望为核糖体及其功能复合物的结构动力学提供基本的见识。

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