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Oleaginous yeast Meyerozyma guilliermondii shows fermentative metabolism of sugars in the biosynthesis of ethanol and converts raw glycerol and cheese whey permeate into polyunsaturated fatty acids

机译:Oleabilicous酵母Meyerozyma Guilliermondii在乙醇的生物合成中显示出发酵的糖代谢,并将原料甘油和奶酪乳清渗透物转化为多不饱和脂肪酸

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Abstract > We studied the biotechnological potential of the recently isolated yeast Meyerozyma guilliermondii BI281A to produce polyunsaturated fatty acids and ethanol, comparing products yields using glucose, raw glycerol from biodiesel synthesis, or whey permeate as substrates. The yeast metabolism was evaluated for different C/N ratios (100:1 and 50:1). Results found that M. guilliermondii BI281A was able to assimilate all tested substrates, and the most efficient conversion obtained was observed using raw glycerol as carbon source (C/N ratio 50:1), concerning biomass formation (5.67?g·L ?1 ) and lipid production (1.04?g·L ?1 ), representing 18% of dry cell weight. Bioreactors experiments under pH and aeration‐controlled conditions were conducted. Obtained fatty acids were composed of ~67% of unsaturated fatty acids, distributed as palmitoleic acid (C <sub>16:1</sub> , 9.4%), oleic acid (C <sub>18:1</sub> , 47.2%), linoleic acid (C <sub> 18:2 n ?6 </sub> , 9.6%), and linolenic acid (C <sub> 18:3 n ?3 </sub> , 1.3%). Showing fermentative metabolism, which is unusual for oleaginous yeasts, M. guilliermondii produced 13.7 g·L ?1 of ethanol (yields of 0.27) when growing on glucose medium. These results suggest the promising use of this uncommonly studied yeast to produce unsaturated fatty acids and ethanol using cheap agro‐industrial residues as substrates in bioprocess. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译:</span><Abstract Type =“Main”XML:Lang =“en”> <标题类型=“main”>抽象</ title> > 我们研究了最近孤立酵母的生物技术潜力 meyerozyma guilliermondii </ i> Bi281a生产多阳饱和脂肪酸和乙醇,比较产物产量使用葡萄糖,从生物柴油合成的生物柴油合成的原料甘油,或作为基材的乳清渗透物。评估酵母代谢的不同C / N比(100:1和50:1)。结果发现了 m。 Guilliermondii </ i> Bi281a能够同化所有测试的底物,并且使用原料甘油作为碳源(C / N比50:1)观察最有效的转化,关于生物质形成(5.67?G·L. ?1 </ sup> )和脂质生产(1.04?G·L. ?1 </ sup> ),代表干细胞重量的18%。进行pH和曝气控制条件下的生物反应器实验。得到的脂肪酸由〜67%的不饱和脂肪酸组成,分布为棕榈酸酸(c <sub> 16:1 </ sub> ,9.4%),油酸(C <sub> 18:1 </ sub> ,47.2%),亚油酸(c <sub> 18:2 n </ i> ?6 </ sub> ,9.6%)和亚麻酸(c <sub> 18:3 n </ i> ?3 </ sub> ,1.3%)。显示发酵代谢,这对于烯胺酵母而言是不寻常的, m。 Guilliermondii </ i> 制作13.7 g·l ?1 </ sup> 在葡萄糖介质上生长时,乙醇(0.27)的产率。这些结果表明,有希望使用这种罕见的酵母在生物过程中使用廉价农业工业残留物生产不饱和脂肪酸和乙醇。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第6期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fabricio Mariana Fensterseifer&option=202" target="_blank" rel="nofollow">Fabricio Mariana Fensterseifer;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Valente Patricia&option=202" target="_blank" rel="nofollow">Valente Patricia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Záchia Ayub Marco Ant?nio&option=202" target="_blank" rel="nofollow">Záchia Ayub Marco Ant?nio;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Biotechnology Laboratory (BiotecLab) of Food Science and Technology InstituteFederal University of Rio Grande do SulPorto Alegre Brazil;</p> <p>Department of Microbiology Immunology and ParasitologyFederal University of Rio Grande do SulPorto Alegre Brazil;</p> <p>Biotechnology Laboratory (BiotecLab) of Food Science and Technology InstituteFederal University of Rio Grande do SulPorto Alegre Brazil;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ethanol production&option=203" rel="nofollow">ethanol production;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microbial lipids&option=203" rel="nofollow">microbial lipids;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=PUFA&option=203" rel="nofollow">PUFA;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=raw glycerol&option=203" rel="nofollow">raw glycerol;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=whey permeate&option=203" rel="nofollow">whey permeate;</a> </p> <div class="translation"> 机译:乙醇生产;微生物脂质;PUFA;生甘油;乳清渗透; 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