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首页> 外文期刊>Biotechnology Progress >Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest in Agave L. for genetic improvement and biocatalysis
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Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest in Agave L. for genetic improvement and biocatalysis

机译：在龙舌兰L中的发酵，生物能源，纤维和生物技术利益生物聚合物基因分析和生物聚合物基因的审查和杀菌改善和生物分析

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摘要

Several of the over 200 known species of Agave L. are currently used for production of distilled beverages and biopolymers. The plants live in a wide range of stressful environments as a result of their resistance to abiotic stress (drought, salinity, and extreme temperature) and pathogens, which gives the genus potential for germplasm conservation and biotechnological applications that may minimize economic losses as a result of the global climate change. However, the limited knowledge in the genus of genome structure and organization hampers development of potential improved biotechnological applications by means of genetic manipulation and biocatalysis. We reviewed Agave and plant sequences in the GenBank NCBI database for identifying genes with biotechnological potential for fermentation, bioenergy, fiber improvement, and in vivo plant biopolymer production. Three-dimensional modeling of enzyme structures in plant accessions revealed structural differences in sucrose 1-fructosyltransferase, fructan 1-fructosyltransferase, fructan exohydrolase (1-FEH), cellulose synthase (CES), and glucanases (EGases) with possible effects in fructan, sugar, and biopolymer production. Although the coding genes of FEH and enzymes involved in biopolymer production (CES, sucrose synthase, and EGases) remain unidentified in Agave L., our results could aid isolation of such genes in Agave. By comparing nucleotide and amino acid sequences in accessions of Agave and other plants, knowledge may be gained about transcriptional regulation and enzymatic activity factors. Future study is needed of biotechnological application of Agave genes for crop breeding aided by genetic engineering and biocatalysis. (c) 2018 American Institute of Chemical Engineers Biotechnol.
机译：超过200种已知的龙舌兰L.目前用于生产蒸馏饮料和生物聚合物。由于它们对非生物胁迫（干旱，盐度和极端温度）和病原体的抵抗力，该植物在各种压力环境中生活在各种压力环境中，这给出了可能最大限度地减少经济损失的种质保存和生物技术应用的潜力全球气候变化。然而，基因组结构和组织属的知识有限，通过遗传操作和生物分析妨碍了潜在改善的生物技术应用的发展。我们审查了Genbank NCBI数据库中的龙舌兰和植物序列，用于鉴定具有发酵，生物能源，纤维改善和体内植物生物聚合物生产的生物技术潜力的基因。植物中酶结构的三维建模揭示了蔗糖1 - 果糖转移酶，果糖1-果糖转移酶，果糖exohydroolase（1-FeH），纤维素合酶（CES）和葡聚糖酶（EGAse）的结构差异，在果脯中，糖和生物聚合物生产。尽管参与生物聚合物生产（CES，蔗糖合酶和EGAsease）的FEH和酶的编码基因仍然不确定，但我们的结果可以帮助在龙舌兰中分离这些基因。通过将核苷酸和氨基酸序列进行比较龙舌兰和其他植物的含量，可以获得关于转录调控和酶活性因子的知识。通过基因工程和生物分析需要对农作物育种的作物育种的生物技术应用未来的研究。 （c）2018美国化学工程师学院Biotechnol。

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