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首页> 外文期刊>Biotechnology Progress >Microbial Lipid Extraction from Lipomyces starkeyi Using Irreversible Electroporation
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Microbial Lipid Extraction from Lipomyces starkeyi Using Irreversible Electroporation

机译：使用不可逆电穿孔的微生物脂质提取血红蛋状胃窦

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The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field. The forces of the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the distance of 2 cm, which provided high treatment intensity (36.7 kWh m(-3)). At this condition, maximum lipid (63 mg g(-1)) was extracted when the biomass was treated for 10 min. During the comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8% for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the time and solvent usage for lipid extraction from microbial biomass. (C) 2018 American Institute of Chemical Engineers
机译：该研究的目的是探讨使用不可逆电穿孔（EP）作为微生物细胞破坏技术的可行性，以在短时间内提取细胞内脂质并以生态友好的方式提取细胞内脂质。设计和制造了EP电路，以获得4 kV，频率为100 Hz的方波。通过EP处理Lipomyces Starkeyi（L. Starkeyi）的酵母细胞2-10分钟，其中电极之间的距离保持在2,4和6cm。计数菌落形成单元（CFU）以观察高压电场下的细胞活力。脉冲电场的力导致L.Starkeyi的细胞壁损伤，并且通过现场发射扫描电子显微镜（FeSEM）图像可视化微生物细胞的破坏。在断开细胞壁后，提取并测量脂质以评估EP以其他技术的效率。当将电极放置在2cm的距离处提供高处理强度（36.7kWh m（-3））时，细胞失活的程度高达95％。在这种情况下，当将生物质处理10分钟时，提取最大脂质（63mg g（-1））。在比较期间，EP可以提取31.88％的脂质，而超声的量为11.89％，对于Fenton的试剂为16.8％。结果建议，EP是一种有希望的技术，用于降低脂质萃取从微生物生物质的时间和溶剂用途。 （c）2018美国化学工程研究所

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