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Stability/activity features of the main enzyme components of rohapect 10L

机译：Rohapect 10L的主要酶组分的稳定性/活性特征

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Abstract >Rohapect 10L is an enzyme cocktail commercialized for juice clarification. Here, we characterized the activity and stability of five enzymatic activities present in this cocktail: total pectinase (PE), polygalacturonase (PG), pectin lyase (PL), pectin methyl esterase (PME), and total cellulase (CE) activities. All these enzyme activities have the maximum activity and stability at pH 4, conditions near those found in most fruit juices. However, if the enzymes need to be handled under different conditions (e.g., to immobilize them), their stability becomes extremely low in some cases, just at pH values slightly higher than the optimal one. For example, at pH 10 only CE was reasonably stable at 25°C, while many other enzyme activities were rapidly almost inactivated, even at 4°C. For these cases, different additives were evaluated, and we found that polyethylene glycol was positive or very positive for all enzyme stabilities, allowing keeping reasonable activities after several hours at pH 10 and 25°C. Another additive, that is, dextran, has a small positive effect for PE, PG, and CE, and a very positive effect for PL, albeit significantly destabilizing PME. Thus, the handling and use of this extract requires some care when is performed out of optimal conditions. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > rohapect 10l是商业化的酶鸡尾酒，用于果汁澄清。在此，其特征在于该鸡尾酒中存在的五种酶活性的活性和稳定性：总果胶酶（PE），聚半乳糖酸酯酶（PG），果胶裂解酶（PL），果胶甲酯酶（PME）和全纤维素酶（CE）活性。所有这些酶活性在pH 4时具有最高的活性和稳定性，在大多数果汁中发现的条件附近。然而，如果需要在不同条件下处理酶（例如，以固定它们），在某些情况下，它们的稳定性变得极低，仅在pH值略高于最佳的pH值。例如，在pH10中，仅在25℃下只能稳定Ce，而甚至在4℃下也迅速地灭活许多其他酶活性。对于这些情况，评估了不同的添加剂，我们发现，对于所有酶稳定性，聚乙二醇是阳性的或非常阳性的，允许在pH10和25℃下几小时保持合理的活性。另一种添加剂，即葡聚糖，对PE，PG和Ce具有小的阳性作用，以及对PL的非常积极的影响，尽管显着稳定的PME。因此，当在最佳条件下执行此提取物的处理和使用需要一些小心。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第6期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dal Magro Lucas&option=202" target="_blank" rel="nofollow">Dal Magro Lucas;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kornecki Jakub F.&option=202" target="_blank" rel="nofollow">Kornecki Jakub F.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Klein Manuela P.&option=202" target="_blank" rel="nofollow">Klein Manuela P.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rodrigues Rafael C.&option=202" target="_blank" rel="nofollow">Rodrigues Rafael C.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fernandez‐Lafuente Roberto&option=202" target="_blank" rel="nofollow">Fernandez‐Lafuente Roberto;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Biocatalysis ICP‐CSICCampus UAM‐CSIC CantoblancoMadrid ZC Spain;</p> <p>Department of Biocatalysis ICP‐CSICCampus UAM‐CSIC CantoblancoMadrid ZC Spain;</p> <p>Department of NutritionFederal University of Health Sciences of Porto Alegre (UFCSPA)Porto Alegre RS Brazil;</p> <p>Biotechnology Bioprocess and Biocatalysis GroupInstitute of Food Science and Technology Federal University of Rio Grande do SulPorto Alegre RS Brazil;</p> <p>Department of Biocatalysis ICP‐CSICCampus UAM‐CSIC CantoblancoMadrid ZC Spain;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cellulosic enzymes&option=203" rel="nofollow">cellulosic enzymes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzyme cocktail activity&option=203" rel="nofollow">enzyme cocktail activity;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzyme cocktail stability&option=203" rel="nofollow">enzyme cocktail stability;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=pectinolytic enzymes&option=203" rel="nofollow">pectinolytic enzymes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=stabilization of enzyme activities&option=203" rel="nofollow">stabilization of enzyme activities;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=stabilizing additives&option=203" rel="nofollow">stabilizing additives;</a> </p> <div class="translation"> 机译：纤维素酶;酶鸡尾酒活动;酶鸡尾酒稳定性;果胶溶解酶;酶活性稳定;稳定添加剂; 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W. </a> <span>2012</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：嗜热古菌中的多酶复合物：温度对多组分体系中稳定性，催化作用和酶相互作用的影响。</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_jiangsu-agricultural-sciences_thesis/020123330850.html">龟裂链霉菌发酵液活性组分SN06对番茄主要防御酶系的影响</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘秋&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘秋</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘长建&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘长建</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王艳颖&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王艳颖</a> <span> <a href="/journal-cn-7304/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 江苏农业科学 </a> </span> <span> . 2004</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_edible-fungi_thesis/0201260007404.html">鸡腿菇纤维素酶系组分活性的特征研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李焱&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李焱</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=孙凌燕&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,孙凌燕</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张蕙蕴&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张蕙蕴</a> <span> <a href="/journal-cn-6950/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 食用菌 </a> </span> <span> . 2009</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_food-science_thesis/020124306284.html">果胶酶ROHAPECT D 5S对猕猴桃取汁和澄清的影响</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘东红&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘东红</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曾超&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,曾超</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王衍彬&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王衍彬</a> <span> <a href="/journal-cn-9417/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 食品科学 </a> </span> <span> . 2001</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201297716986.html">果胶酶ROHAPECT VR-C对猕猴桃取汁和澄清的影响</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=叶兴乾&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 叶兴乾</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=苏平&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,苏平</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈健初&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈健初</a> <span> <a href="/journal-cn-57200/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 科技通报 </a> </span> <span> . 2001</span><span>,第3期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295957763.html">不同活性组分催化剂的氧化活性和热稳定性</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨竹仙&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨竹仙</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈晓银&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈晓银</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘勇&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘勇</a> <span> <a href="/journal-cn-56690/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 环境科学 </a> </span> <span> . 2000</span><span>,第5期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-60330_thesis/020222695242.html">野生与栽培夏枯草主要活性组分含量特征及质量评价</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=PI Sheng-ling&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . PI Sheng-ling</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=皮胜玲&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,皮胜玲</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ZHANG Kai-qiang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,ZHANG Kai-qiang</a> <span> <a href="/conference-cn-60330/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中华中医药学会第十一届中药化学年会 </a> <span> <span> . 2016</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020313505850.html">荒漠草原沙漠化过程中土壤有机碳活性组分特征及其稳定性</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=阎欣&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 阎欣</a> <span> . 2018</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120104359374.html">用于控制释放活性组分的稳定亲脂基质为主要组分的药物组合物</a> <b>[P]</b> . <span> 中国专利： CN1171270A </span> <span> . 1998-01-28</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120111207537.html">一种以玫瑰提取物为主要活性组分的保湿性护肤品</a> <b>[P]</b> . <span> 中国专利： CN108703915A </span> <span> . 2018-10-26</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130426234342.html">Protein or polypeptide having tearing component-generating enzyme activity, DNA encoding the protein or polypeptide, method for producing protein or polypeptide having tearing component-generating enzyme activity using the DNA, and mRNA for the protein or polypeptide Nucleic acid molecule having a function of inhibiting the translation of</a> <b>[P]</b> . <span> 外国专利：  JP4611638B2 </span> <span> . 2011-01-12</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：具有产生撕裂成分的酶活性的蛋白质或多肽，编码该蛋白质或多肽的DNA，使用该DNA生产具有产生撕裂成分的酶活性的蛋白质或多肽的方法以及该蛋白质或多肽的mRNA具有抑制功能的核酸分子的翻译 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130440626761.html">The mRNA for the polypeptide or protein and method for producing a polypeptide or protein with DNA encoding a polypeptide polypeptide, or protein or protein with tear component generation enzyme activity, tear component generation enzyme activity using the DNA nucleic acid molecule having the ability to inhibit translation of</a> <b>[P]</b> . <span> 外国专利：  JPWO2003074706A1 </span> <span> . 2005-06-30</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：多肽或蛋白质的mRNA以及使用具有编码多肽多肽的DNA的多肽或蛋白质的方法，或具有泪液成分产生酶活性的蛋白质或蛋白质的生产方法，使用具有抑制翻译能力的DNA核酸分子来生产泪液成分产生酶的活性的 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130450070101.html">Raising the pH and temperature stability of enzyme preparations from Trichoderma reesei cultures for use as cellulolytic enzymes, by raising the pH and/or temperature of an aqueous solution of the culture until the enzyme activity falls</a> <b>[P]</b> . <span> 外国专利：  DE19831553A1 </span> <span> . 2000-01-13</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过升高培养物水溶液的pH和/或温度直至酶活性下降，来提高来自里氏木霉（Trichoderma reesei）用作纤维素分解酶的酶制剂的pH和温度稳定性 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div 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