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Behavior of Several Pseudomonas putida Strains Growth Under Different Agitation and Oxygen Supply Conditions

机译：几种假单胞菌的行为在不同搅拌和氧气供应条件下的增长

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The growth rate of four strains of Pseudomonas putida, KT2440, KT2442, KTH2, and KTH2 (pESOX3), under different fluid dynamic conditions has been studied. The cultures were conducted in a stirred tank bioreactor by changing the stirrer speed. Several process variables, such as biomass concentration, dissolved oxygen concentration, oxygen mass transfer rate and oxygen uptake rate, have been measured or calculated. Also cell viability was determined by viable colony counting in Petri dishes and culture samples were subjected into a transmission electron microscopy analysis, in order to describe the integrity of the individual cells. The experimental results show that the genetically modified organisms, the strains KTH2 and KTH2 (pESOX3), present a different growth under low agitation conditions, and low oxygen supply level, while the growth of the wild type strains, KT2440 and KT2442, followed the typical sigmoidal evolution that could be described by the logistic equation. The presence of outer membrane vesicles has been observed in the GMO strains. When the cultures were conducted at low stirrer speed, and so at low oxygen transfer rate, these vesicles were detected, indicating the bacterial response to oxidative stress, caused by the catalytic activity of the HpaC enzyme. For all of the strains tested, no hydrodynamic stress has been detected, even at very high agitation levels. (C) 2018 American Institute of Chemical Engineers
机译：研究了在不同流体动态条件下，研究了普赖达，KT2440，KT242，Kth2和Kth2（Pesox3）的四种菌株的生长速率。通过改变搅拌器速度，在搅拌釜生物反应器中进行培养物。已经测量或计算了几种过程变量，例如生物质浓度，溶解的氧浓度，氧气传质速率和氧吸收率。通过在培养皿中的可行性菌落测定细胞活力，并且将培养样品进行培养物电子显微镜分析，以描述个体细胞的完整性。实验结果表明，遗传修饰的生物，菌株Kth2和Kth2（Pesox3），在低搅拌条件下具有不同的生长，低氧气供应水平，而野生型菌株的生长，KT2440和KT2442，典型物流方程可以描述的符号演变。已经在GMO菌株中观察到外膜囊泡的存在。当以低搅拌器速度进行培养物，在低氧转移速率下，检测这些囊泡，表明由HPAC酶的催化活性引起的细菌反应。对于所有测试的菌株，即使在非常高的搅动水平下也没有检测到流体动力学应激。 （c）2018美国化学工程研究所

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