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首页> 外文期刊>Biotechnology Progress >Optimized immobilization of endoglucanase Cel9A onto glutaraldehyde activated chitosan nanoparticles by response surface methodology: The study of kinetic behaviors
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Optimized immobilization of endoglucanase Cel9A onto glutaraldehyde activated chitosan nanoparticles by response surface methodology: The study of kinetic behaviors

机译：通过响应面方法优化了内葡聚糖酶CEL9a在戊二醛活化的壳聚糖纳米粒子上的固定化：动力学行为研究

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摘要

Immobilization of enzyme onto nanoparticles such as chitosan can have biotechnological importance. In this study, chitosan nanoparticles (ChNPs) were prepared by Ionic gelation method and Endoglucanase Cel9A from Alicyclobacillus acidocaldariius (AaCel9A) immobilized on the nanoparticles. The FTIR results showed that the enzymes were immobilized on the ChNPs. The dynamic light scattering and scanning electron microscope (SEM) results illustrated that the AaCel9A-ChNPs approximately had 40 nm diameters. For optimizing enzyme immobilization, response surface methodology was employed using different variables (pH, enzyme immobilization time, and enzyme to ChNPs ratio [E/Cs]). The results showed that the high immobilization efficiency was achieved in pH 7, E/Cs of 0.4 in 2.63 hr. The enzyme activity results showed that, immobilization increased optimum pH for activity (from 6.5 to 7.5) and the enzyme K-m (from 3.703 to 12.195 [mg/ml]), which make it suitable to use in some industries such as detergents.
机译：将酶固定到纳米颗粒上，例如壳聚糖可以具有生物技术重要性。在该研究中，通过离子凝胶法和内葡聚糖酶CEL9a制备壳聚糖纳米颗粒（CHNP），从固定在纳米颗粒上的alicclobacillus acidocaldariius（aacel9a）中的内葡聚糖酶Cel9a制备。 FTIR结果表明将酶固定在CHNP上。动态光散射和扫描电子显微镜（SEM）结果示出了Aacel9a-Chnps大约具有40nm的直径。为了优化酶固定化，使用不同变量（pH，酶固定时间和酶至CHNPS比[E / CS]）使用响应面方法。结果表明，在2.63小时内为0.4的0.4的高固定效率。酶活性结果表明，固定化对活性（从6.5至7.5）和酶K-M（从3.703-12.195 [Mg / ml]）增加，这使得适用于某些行业，如洗涤剂。

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