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首页> 外文期刊>Biotechnology Progress >A Simple Cell Transport Device Keeps Culture Alive and Functional During Shipping
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A Simple Cell Transport Device Keeps Culture Alive and Functional During Shipping

机译：一个简单的细胞传输装置在运输过程中保持文化和功能

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Transporting living complex cellular constructs through the mail while retaining their full viability and functionality is challenging. During this process, cells often suffer from exposure to suboptimal life-sustaining conditions (e.g. temperature, pH), as well as damage due to shear stress. We have developed a transport device for shipping intact cell/tissue constructs from one facility to another that overcomes these obstacles. Our transport device maintained three different cell lines (Caco2, A549, and HepG2 C3A) individually on transwell membranes with high viability (above 97%) for 48 h under simulated shipping conditions without an incubator. The device was also tested by actual overnight shipping of blood brain barrier constructs consisting of human induced pluripotent brain microvascular endothelial cells and rat astrocytes on transwell membranes to a remote facility (approximately 1200 miles away). The blood brain barrier constructs arrived with high cell viability and were able to regain full barrier integrity after equilibrating in the incubator for 24 h; this was assessed by the presence of continuous tight junction networks and in vivo-like values for trans-endothelial electrical resistance (TEER). These results demonstrated that our cell transport device could be a useful tool for long-distance transport of membrane-bound cell cultures and functional tissue constructs. Studies that involve various cell and tissue constructs, such as the "Multi-Organ-on-Chip" devices (where multiple microscale tissue constructs are integrated on a single microfluidic device) and studies that involve microenvironments where multiple tissue interactions are of interest, would benefit from the ability to transport or receive these constructs. (C) 2017 American Institute of Chemical Engineers
机译：通过邮件运输生活复杂的蜂窝结构，同时保留完全活力和功能是具有挑战性的。在此过程中，细胞通常遭受暴露于次优寿命维持条件（例如温度，pH），以及由于剪切应力引起的损坏。我们开发了一种用于将完整的细胞/组织结构从一个设施运送到另一个克服这些障碍的运输装置。我们的运输装置在具有高活力（高于97％）的Transwell膜上，在没有培养箱的模拟的运输条件下，在Transwell膜上单独地保持三种不同的细胞系（CaCO 2，A549和HepG2 C3A）。该器件还通过实际过夜运输血脑屏障构建体，由人类诱导的多能脑微血管内皮细胞和Transwell膜上的大鼠星形胶质细胞组成的血脑屏障构建体进行测试到远程设施（约1200英里远）。血脑屏障构建体具有高细胞活力，并且能够在培养箱中平衡24小时后重新获得全屏完整性;这是通过连续紧密结网络的存在和体内值的跨内皮电阻（TEER）进行评估。这些结果表明，我们的细胞传输装置可以是膜结合细胞培养物的长距离传输和功能组织构建体的有用工具。涉及各种细胞和组织构建体的研究，例如“多器材片”装置（其中多种微型组织构建体集成在单个微流体装置上）和涉及多种组织相互作用感兴趣的微环境的研究，将受益于运输或接收这些构造的能力。 （c）2017美国化学工程师研究所

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