• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Assembly of Breast Cancer Heterotypic Spheroids on Hyaluronic Acid Coated Surfaces
【24h】

Assembly of Breast Cancer Heterotypic Spheroids on Hyaluronic Acid Coated Surfaces

机译：透明质酸涂层表面上的乳腺癌异质型球体组装

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Drug screening is currently demanding for realistic models that are able to reproduce the structural features of solid tumors. 3D cell culture systems, namely spheroids, emerged as a promising approach to provide reliable results during drug development. So far, liquid overlay technique (LOT) is one of the most used methods for spheroids assembly. It comprises cellular aggregation due to their limited adhesion to certain biomaterials, like agarose. However, researchers are currently improving this technique in order to obtain spheroids on surfaces that mimic cancer extracellular matrix (ECM), since cell-ECM interactions modulate cells behavior and their drug resistance profile. Herein, hyaluronic acid (HA) coated surfaces were used, for the first time, for the production of reproducible heterotypic breast cancer spheroids. The obtained results revealed that it is possible to control the size, shape, and number of spheroids gotten per well by changing the HA concentration and the number of cells initially seeded in each well. (C) 2017 American Institute of Chemical Engineers
机译：药物筛选目前要求能够再现实体肿瘤的结构特征的现实模型。 3D细胞培养系统即球状体，作为在药物开发期间提供可靠的结果的有希望的方法。到目前为止，液体覆盖技术（批次）是球形组件最使用的方法之一。它包括由于它们与某些生物材料的有限粘附而导致的细胞聚集，如琼脂糖。然而，研究人员目前正在改进该技术，以便在模拟癌细胞基质（ECM）的表面上获得球状体，因为细胞-ECM相互作用调节细胞行为及其耐药性。在此，首次使用透明质酸（HA）涂覆的表面，用于生产可重复的异质乳腺癌球状体。所得结果显示，通过改变HA浓度和每个孔最初播种的细胞数量，可以控制每个孔的尺寸，形状和数量。 （c）2017美国化学工程师研究所

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号