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Examining the role of particle size on ammonia-based bioprocessing of maize stover

机译：检查粒度对玉米秸秆生物处理的作用

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The role of particle size in carbohydrate fractionation upon pretreatment and glucan yields upon enzymatic hydrolysis was investigated at two different temperatures, to examine the possibility of pretreating under milder conditions smaller particles, in order to satisfy pilot-scale operational constraints. Maize stover was knife-milled through 1-mm and 0.5-mm screens and pretreated by soaking in aqueous ammonia pretreatment at 60 or 110 degrees C for 6 h. Pretreated solids were analyzed for composition and a material balance calculated for glucan, xylan, and lignin. At 60 degrees C, milling resulted in greater delignification compared to unmilled biomass. Delignification was more uniform at 110 degrees C. Pretreated solids were washed and cellulase hydrolysis carried out at 10% w/w solids loading, with low and high enzyme loadings. Liquid samples were drawn and concentration data developed through HPLC to calculate 48-h glucan and xylan hydrolytic yields. The differences in hydrolytic yield between milled and unmilled treatments were found to vary with pretreatment temperature and enzyme loading. The results show that while particle size impacts carbohydrate recovery and hydrolytic yield, it is less important in bioprocessing than pretreatment temperature and enzyme loading, possibly owing to the particles' morphology rather than the size. (c) 2015 American Institute of Chemical Engineers Biotechnol. Prog., 32:134-140, 2016
机译：在两个不同的温度下研究了在预处理和葡聚糖产量对酶水解时进行预处理和葡聚糖产量的作用，以检查在较温和条件下较小的颗粒的预处理的可能性，以满足先导规模的操作系统。玉米秸秆通过1毫米和0.5毫米的筛网进行刀磨，并通过浸泡在60或110℃的氨水预处理中进行预处理6小时。分析预处理的固体用于组合物和为葡聚糖，Xylan和木质素计算的物质平衡。在60摄氏度下，与未磨化的生物质相比，研磨导致更大的脱野。在110℃下脱铝更均匀。洗涤预处理的固体并在10％w / w固体负载下进行，具有低和高酶载荷的纤维素酶水解。绘制液体样品并通过HPLC开发的浓缩数据，以计算48-H葡聚糖和木聚糖水解产率。发现研磨和未磨碎的处理之间水解产率的差异随预处理温度和酶负载而变化。结果表明，虽然粒径会影响碳水化合物回收和水解产率，但在生物加工方面比预处理温度和酶负载不太重要，可能由于颗粒的形态而不是尺寸。 （c）2015美国化学工程研究所生物科技。 Prog。，32：134-140，2016

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