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首页> 外文期刊>Biotechnology Progress >Glucose monitoring and adaptive feeding of mammalian cell culture in the presence of strong autofluorescence by near infrared Raman spectroscopy
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Glucose monitoring and adaptive feeding of mammalian cell culture in the presence of strong autofluorescence by near infrared Raman spectroscopy

机译：近红外拉曼光谱法在强大的自发荧光存在下哺乳动物细胞培养的葡萄糖监测和适应性

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Raman spectroscopy offers an attractive platform for real-time monitoring and control of metabolites and feeds in cell culture processes, including mammalian cell culture for biopharmaceutical production. However, specific cell culture processes may generate substantial concentrations of chemical species and byproducts with high levels of autofluorescence when excited with the standard 785 nm wavelength. Shifting excitation further toward the near-infrared allows reduction or elimination of process autofluorescence. We demonstrate such a reduction in a highly autofluorescent mammalian cell culture process. Using the Kaiser RXN2-1000 platform, which utilizes excitation at 993 nm, we developed multivariate glucose models in a cell culture process which was previously impossible using 785 nm excitation. Additionally, the glucose level in the production bioreactor was controlled entirely by Raman adaptive feeding, allowing for maintenance of glucose levels at an arbitrary set point for the duration of the culture. (c) 2018 American Institute of Chemical Engineers
机译：拉曼光谱提供了一种有吸引力的平台，用于实时监测和控制代谢物，在细胞培养方法中提供，包括用于生物制药生产的哺乳动物细胞培养物。然而，当用标准的785nm波长激发时，特定细胞培养方法可以产生具有高水力的化学物质和具有高水力的副产品。进一步向近红外移动激发允许减少或消除过程自发荧光。我们展示了这种高度自过荧光哺乳动物细胞培养过程的降低。使用KAISER RXN2-1000平台，在993nm下使用激发，我们在以前不可能使用785nm激发的细胞培养过程中开发了多变量葡萄糖模型。另外，生产生物反应器中的葡萄糖水平完全通过拉曼自适应进料来控制，允许在培养的持续时间内维持在任意设定点处的葡萄糖水平。 （c）2018美国化学工程研究所

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