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首页> 外文期刊>Biotechnology Progress >Covalent immobilization of halohydrin dehalogenase for efficient synthesis of epichlorohydrin in an integrated bioreactor
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Covalent immobilization of halohydrin dehalogenase for efficient synthesis of epichlorohydrin in an integrated bioreactor

机译：卤代醇脱卤酶的共价固定化综合生物反应器中表氯醇的高效合成

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Halohydrin dehalogenase (HHDH)-mediated dehalogenation of 1,3-dichloro-2-propanol (1,3-DCP) is a key step in the chemoenzymatic synthesis of epichlorohydrin (ECH) from glycerol. In this study, a covalent immobilization strategy was employed to enhance the stability of Agrobacterium tumefaciens HHDH using epoxy resin ES-103B as a carrier. Under optimal conditions, the activity recovery of ES-103B-immobilized HHDH (HHDH@ES-103B) was 62.4% and the specific activity was 1604 U/g. The HHDH@ES-103B exhibited excellent thermostability, with a half-life of 68.6 days at 40 degrees C, which is 8.0-times higher than that of the free HHDH. A semicontinuous biotransformation of 1,3-DCP to ECH was performed using HHDH@ES-103B as biocatalyst in a recirculating packed bed reactor (RPBR), resulting in an ECH yield of 94.2%, with an average productivity of 5.2 g/L/h. The RPBR system exhibited a high operational stability and even after 50 cycles of reaction, it retained90% of the initial conversion. Furthermore, an integrated bioprocess based on in situ product recovery (ISPR) was developed in RPBR to overcome product inhibition. The integrated bioreactor equipped with an external macroporous adsorption resin HZD-9 column led to another 1.6-fold increase in ECH productivity to 8.46 g/L/h. This improved stability and reusability of HHDH@ES-103B demonstrated its potential for the biotransformation of 1,3-DCP to ECH. (c) 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:784-792, 2018
机译：卤代氢化酶（HHDH）介导的1,3-二氯-2-丙醇（1,3-DCP）的脱卤是脱卤素，是从甘油中的表氯醇（ECH）的化学酶合成的关键步骤。在该研究中，使用共价固定策略来增强Tumefaciens HHDH使用环氧树脂ES-103B作为载体的肿瘤杆菌HHDH的稳定性。在最佳条件下，ES-103B-固定化HHDH（HHDH @ ES-103B）的活性回收率为62.4％，比活性为1604 u / g。 HHDH @ ES-103B表现出优异的热稳定性，半衰期为68.6天，40℃，比自由HHDH高8.0倍。使用HHDH @ ES-103B在再循环填充床反应器（RPBR）中作为生物催化剂进行1,3-DCP至ECH的半连续生物转化，得到94.2％的ECH产率，平均生产率为5.2g / L / H。 RPBR系统表现出高的操作稳定性，即使在50次反应循环后，它也保留了初始转化的90％。此外，在RPBR中开发了基于原位产品回收（ISPR）的一体化生物处理，以克服产品抑制。配备外部大孔吸附树脂HZD-9柱的集成生物反应器LED升至ECH生产率的另一个增加1.6倍至8.46克/升/小时。这种改进的HHDH @ ES-103B的稳定性和可重用性证明了1,3-DCP的生物转化率为ECH的潜力。 （c）2018美国化学工程师学院Biotechnol。 Prog。，34：784-792,2018

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